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环境化学  2015 

采用尼罗红荧光探针对微藻中油脂的定量测定

Keywords: 尼罗红,油脂,微藻,定量

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Abstract:

以尼罗红为荧光探针,对小球藻油脂含量的定量分析条件进行了研究.测定中,使用48孔酶标仪作为荧光检测器.研究显示,当荧光激发光波长为485nm,发射光波长为580nm时,适用于测定微藻中的中性油脂含量.当二甲基亚砜终浓度为20%,尼罗红终浓度为0.5μg·mL-1,藻液与尼罗红置于40℃暗处反应5min后,能够获得最大的相对荧光测定值.当藻液光密度OD680在0.61至1.73时,藻液油脂含量与尼罗红荧光测定值呈现良好的线性关系,相关系数R2为0.9836,可以通过尼罗红荧光值的测定推算油脂的含量.

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