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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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阴离子聚电解质接枝微球CPVA-g-PSSS的制备及其对碱性蛋白吸附特性的研究
DOI: 10.11777/j.issn1000-3304.2015.15139, PP. 1353-1362
Keywords: 交联聚乙烯醇微球,对苯乙烯磺酸钠,聚电解质接枝刷,碱性蛋白质,吸附机理
Abstract:
通过分子设计,将大量可聚合双键引入交联聚乙烯醇(CPVA)微球表面,然后采用"穿过"法("graftingthrough"method)将对苯乙烯磺酸钠(SSS)接枝于CPVA微球表面,制备了具有阴离子聚电解质接枝刷状结构的接枝微粒CPVA-g-PSSS.采用红外光谱(FTIR)和扫描电子显微镜(SEM)对接枝微粒进行了表征,使用zeta电位分析仪测定了接枝微粒zeta电位曲线.将接枝微粒CPVA-g-PSSS用于对胰蛋白酶(TRY)和溶菌酶(LZM)2种碱性蛋白的吸附,考察了主要因素对吸附性能的影响,探讨了吸附机理,研究了吸附热力学.研究结果表明,采用本研究的方法,可顺利的实现阴离子单体SSS的接枝聚合.在强静电相互作用的驱动下,阴离子聚电解质接枝刷CPVA-g-PSSS对2种碱性蛋白表现出很强的吸附能力.介质的pH值对吸附容量有很大的影响,随着介质pH值的增大,TRY和LZM的吸附容量均呈现先增大后减小的变化趋势,当pH值等于它们的等电点时,具有最高的吸附容量(TRY,82mg/g;LZM,78mg/g).以2种碱性蛋白等电点为界,离子强度对吸附容量产生完全相反的影响作用,当介质pH值等于它们等电点时,吸附容量几乎不随电解质的浓度发生变化.吸附是一放热过程,且使体系的熵值增大.
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