|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
采用分子表面印迹技术构建手性空穴实现对天冬氨酸对映体的识别拆分
DOI: 10.3724/SP.J.1105.2012.11061, PP. 47-55
Keywords: 天冬氨酸,手性拆分,分子表面印迹,4-乙烯基吡啶,接枝聚合,分子识别
Abstract:
在溶液聚合体系中,采用"接出"法("graftfrom"method)将功能单体4-乙烯基吡啶(4VP)接枝聚合于微米级硅胶表面,制得了功能接枝微粒P4VP/SiO2,采用本课题组建立的新型分子表面印迹技术,以天冬氨酸(Asp)的一种对映体L-Asp为模板分子,二溴己烷为交联剂,对接枝在硅胶表面的大分子P4VP实施了分子印迹,制备了L-Asp分子表面印迹材料MIP-P4VP/SiO2.以天冬氨酸另一种对映体D-Asp为对比物,采用静态与动态两种方法深入研究了MIP-P4VP/SiO2对L-Asp分子的识别性能,重点探索了采用MIP-P4VP/SiO2对天冬氨酸两种对映体进行拆分的可行性.实验结果表明,印迹材料MIP-P4VP/SiO2对模板分子L-Asp具有良好的识别选择性与结合亲和性,相对于D-Asp,识别选择性系数为3.85,对天冬氨酸的外消旋体具有良好的拆分性能.印迹材料MIP-P4VP/SiO2也具有良好的解吸性能,以稀NaOH水溶液作为洗脱液,12个床体积内解吸率即可达到98.52%.
| [1] | 2 Yoshikawa M,Murakoshi K,Kogita T,Hanaoka K,Guiver M D,Robertson G P.Eur Polym J,2006,42:2532~2539
|
| [2] | 3 Higuchi A,Hayashi A,KandaN,Sanui K,Kitamur H.J Mol Struct,2005,739:145~152
|
| [3] | 4 Dimitrova P,Bart H J.Anal Chim Acta,2010,663:109~116
|
| [4] | 5 Sanaie N,Haynes C A.J Chromatogr A,2006,1132:39~50
|
| [5] | 7 André C,Thomassin M,Umrayamib A,Ismaili L,Refouvelet B,Guillaume Y C.Talanta,2007,71:1817~1823
|
| [6] | 9 Hdl H,Schmid M G,Gübitz G.J Chromatogr A,2008,1204:210~218
|
| [7] | 13 Steensma M,Kuipers N J M,Haan A B de,Kwant G.Chem Eng Sci,2007,62:1395~ 1407
|
| [8] | 14 Francotte E R.J Chromatogr A,2001,906:379~397.
|
| [9] | 16 Javanbakhta M,Fard S E,Mohammadi A,Abdouss M,Ganjali M R,Norouzi P,Safaraliee L.Anal Chim Acta,2008,612:65~74
|
| [10] | 17 Claude B,Morin P,Lafosse M,Belmont A S,Haupt K.Talanta,2008,75:344~350
|
| [11] | 18 Dias A C B,Figueiredo E C,Grassi V,Zagatto E A G,Arruda M A Z.Talanta,2008,76:988~996
|
| [12] | 19 Li M,Lin X C,Xie Z H.J Chromatogr A,2009,1216:5320~5326
|
| [13] | 20 Lu Y,Li C X,Liu X H,Huang W Q.J Chromatogr A,2002,950:89~97
|
| [14] | 21 Wang Y T,Zhou Y X,Sokolov J,Rigas B,Levon K,Rafailovich M.Biosens Bioelectron,2008,24:162~166
|
| [15] | 22 Liu H M,Liu C H,Yang X J,Zeng S J,Xiong Y Q,Xu W J.Anal Chim Acta,2008,628:87~94
|
| [16] | 23 Luo W,Zhu L H,Yu C,Tang H Q,Yu H X,Li X,Zhang X.Anal Chim Acta,2008,618:147~156
|
| [17] | 24 Zhang Zhaohui(张朝晖),Liu Li(刘丽),Nie Lihua(聂丽华).Acta Polymerica Sinica(高分子学报),2010,(6):677~683
|
| [18] | 25 Gao B J,Lu J H,Chen Z P,Guo J F.Polymer,2009,50:3275~3284
|
| [19] | 26 Gao B J,Wang J,An F Q,Liu Q.Polymer,2008,49:1230~1238
|
| [20] | 27 Gao B J,Liu S Y,Li Y B.J Chromatogr A,2010,1217:2226~2236
|
| [21] | 28 Gao B J,Niu Q Y,Du R K.J Sep Sci,2010,33:1338~1348
|
| [22] | 31 Ers？z A,Say R,Denizli A.Anal Chim Acta,2004,502:91~97
|
| [23] | 1 Itou Y, Nakano M,Yoshikawa M.J Membrane Sci,2008,325:371~375
|
| [24] | 6 Bertrand,Chabin A,Brack A,Westall F.J Chromatogr A,2008,1180:131~137
|
| [25] | 8 Tran C D,Kang J.J Chromatogr A,2002,978:221~230
|
| [26] | 10 Natalini B,Sardella R,Macchiarulo A,Pellicciari R.J Chromatogr B,2008,875:108~117
|
| [27] | 11 Sanaie N,Haynes C A.J Chromatogr A,2006,1104:164~172
|
| [28] | 12 Tan B,Luo G S,Wang J D.Sep Purifi Technol,2007,53:330~336
|
| [29] | 15 Bergmann N M,Peppas N A.Prog Polym Sci,2008,33:271~288
|
| [30] | 29 Gao Xuechao(高学超),Gao Baojiao(高保娇),Niu Qingyuan(牛庆媛),Zhao Jing(赵婧).Acta Chemica Sinica(化学学报),2010,68:1109~1118
|
| [31] | 30 Guo Jianfeng(郭建峰),Gao Baojiao(高保娇),An Fuqiang(安富强).Journal of Functional Polymers(功能高分子学报),2009,22:363~368
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
