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温敏性半乳糖基化壳聚糖水凝胶的制备及其对HL-7702细胞增殖行为影响的研究

DOI: 10.3724/SP.J.1105.2013.12311, PP. 888-895

Keywords: 半乳糖基化壳聚糖水凝胶,温度响应性,人正常肝细胞系,细胞脱附,细胞转载

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Abstract:

以壳聚糖(CS)为原料,在1-乙基-3-(3-二甲胺丙基)碳-二亚胺盐酸盐(EDC·HCL)和N-羟基琥珀酰亚胺(NHS)的活化作用下,合成了半乳糖基化壳聚糖(GC)单体,并与N-异丙基丙烯酰胺(NIPAAm)反应,制备了温敏性半乳糖基化壳聚糖N-异丙基丙烯酰胺共聚水凝胶(Gal-CS-g-PNIPAAm).通过红外光谱(FTIR)、光电子能谱(XPS)和扫描电子显微镜(SEM)等测试方法对其成分和结构进行了表征,并对其溶胀率和表面亲疏水性进行了研究.在Gal-CS-g-PNIPAAm凝胶表面培养人正常肝细胞系(HL-7702),研究其生长、脱附及转载(再增殖)行为.结果表明Gal-CS-g-PNIPAAm水凝胶具有良好的温度响应性和生物相容性,与PNIPAAm水凝胶相比,Gal-CS-g-PNIPAAm凝胶表面更有利于HL-7702细胞增殖.将温度降低至临界温度(LCST,32.5℃)以下,细胞可以从凝胶表面自发脱附,与酶消化脱附相比,细胞损伤更少.Gal-CS-g-PNIPAAm凝胶表面脱附的细胞比PNIPAAm凝胶表面脱附的细胞活性更高,表明PNIPAAm水凝胶引入GC单体后,凝胶的生物相容性得到改善,且脱附后细胞的增殖活力明显增加.

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