OALib Journal期刊
ISSN: 2333-9721
费用：99美元

投递稿件

查看量	下载量

相关文章
更多...

化学进展 2008

单细胞分析中的荧光标记化学*

, PP. 2045-2052

朱兰兰,殷学锋

Keywords: 标记化学,细胞内衍生,电穿孔,单细胞分析

Full-Text Cite this paper Add to My Lib

Abstract:

细胞内组分复杂、含量低，因此测定单细胞内化学组分的分析方法必须具有灵敏度高、选择性好和分辨率高的特点。高灵敏度的荧光检测技术是单细胞分析中应用最多的检测方法之一。但是细胞内绝大部分物质其天然态是没有荧光的，且由于细胞膜的阻碍，衍生试剂不能自由地进入细胞内。为了使衍生试剂透过细胞膜标记细胞内待测物质而不引起显著的稀释效应，已进行了大量的研究工作。本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇（PEG）等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。对这些方法的原理、特点和在单细胞分析中的应用也做了较为详细的阐述。

References

[1]	[5 ] Oates M D , Cooper B R , Jorgenson J W. Anal . Chem. , 1990 ,62 : 1573 —1577
[2]	[9 ] Menger F M, Gabrielson K D. Angew. Chem. , 1995 , 107 :2260 —2278
[3]	Hogan B L , Yeung E S. Anal . Chem. , 1992 , 64 : 2841 —2845
[4]	Sun Y, Yin X F. J . Chromatogr. A , 2006 , 1117 : 228 —233
[5]	Orwar O , Fishman H A , Ziv N E , et al . Anal . Chem. , 1995 ,67 : 4261 —4268
[6]	Loetchutinata C , Kothana S , Mankhetkorn S R , et al . Phys.Chem. , 2005 , 72 : 323 —331
[7]	Soh N. Anal . Bioanal . Chem. , 2006 , 386 : 532 —543
[8]	Sun Y, Yin X F , Ling Y Y, Fang Z L. Anal . Bioanal . Chem. ,2005 , 382 : 1472 —1476
[9]	Chang M C Y, Pralle A , Isacoff E Y, Chang CJ . J . Am. Chem.Soc. , 2004 , 126 : 15392 —15393
[10]	Setsukinai K, Urano Y, Nagano T, et al . J . Biol . Chem. , 2003 ,278 : 3170 —3175
[11]	Szücs S , Vámosi G, Póka R , et al . Cytometry , 1998 , 33 : 19 —31
[12]	Yang M, Li C W, Yang J . Anal . Chem. , 2002 , 74 : 3991 —4001
[13]	Wheeler A R , Throndset W R , Daridon A , et al . Anal . Chem. ,2003 , 75 : 3581 —3586
[14]	Cheng W, Klauke N , Cooper J M, et al . Lab Chip , 2006 , 6 :1424 —1431
[15]	Peng X Y, Li P C H. Anal . Chem. , 2004 , 76 : 5282 —5292
[16]	Rocheleau J V , Walker G M, Piston D W, et al . Proc. Natl .Acad. Sci . USA , 2004 , 101 : 12899 —12903
[17]	Li XJ , Li P C H. Anal . Chem. , 2005 , 77 : 4315 —4322
[18]	Walkup G K, Burdette S C , Lippard S J , Tsien R Y. J . Am.Chem. Soc. , 2000 , 122 : 5644 —5645
[19]	Maruyama S , Kikuchi K, Nagano T, et al . J . Am. Chem. Soc. ,2002 , 124 : 10650 —10651
[20]	Taki M, Wolford J L , O’Halloran T V , et al . J . Am. Chem.Soc. , 2004 , 126 : 712 —713
[21]	Komatsu H , Iwasawa N , Citterio D , et al . J . Am. Chem. Soc. ,2004 , 126 : 16353 —16360
[22]	Inglefield J R , Schwartz-Bloom R D. J . Neurochem. , 1998 , 70 :2500 —2509
[23]	Heo J , Thomas K J , Seong G H , Crooks R M A. Anal . Chem. ,2003 , 75 : 22 —26
[24]	Neumann E , Schaefer-Ridder M, Wang Y, Hofschneider P H.EMBO J . , 1982 , 1 : 841 —845
[25]	Khine M, Lau A , Lee L P , et al . Lab Chip , 2005 , 5 : 38 —43
[26]	Wang H Y, Lu C. Anal . Chem. , 2006 , 78 : 5158 —5164
[27]	Agarwal A , Zudans I , Weber S G, et al . Anal . Chem. , 2007 ,79 : 3589 —3596
[28]	Zhang H , Jin W. J . Chromatogr. A , 2006 , 1104 : 346 —351
[29]	Dong Q , Jin W. Electrophoresis , 2001 , 22 : 2786 —2792
[30]	Otake K, Imura T, Sakai H , Abe M. Langmuir , 2001 , 17 :3898 —3901
[31]	Ulrich A S. Biosci . Rep. , 2002 , 22 : 129 —150
[32]	Sun Y, Lu M, Yin X F. Chem. J . Chin. Univ. , 2006 , 27 :632 —634
[33]	Sun Y, Lu M, Yin X F , Gong X G. J . Chromatogr. A , 2006 ,1135 : 109 —114
[34]	Chen F , Zhi D , Xia G. Electrophoresis , 2005 , 26 : 4204 —4205
[35]	Swanek F D , Anderson B B , Ewing A G. J . Microcol . Sep. ,1998 , 10 : 185 —192
[36]	Weng Q , Jin W. Electrophoresis , 2001 , 22 : 2797 —2803
[37]	Oguri S , Ohta Y, Suzuki C. J . Chromatogr. B , 1999 , 736 :263 —271
[38]	Harwood M M, Bleecker J V , Rabinovitch P S , Dovichi N J .Electrophoresis , 2007 , 28 : 932 —937
[39]	凌云扬(Ling Y Y) , 殷学锋( Yin X F) , 方肇伦( Fang Z L) .高等学校化学学报(Chem. J . Chi . Univ. ) , 2005 , 26 : 247 —249
[40]	Lochmann H , Bazzanella A , B?c hmann K. J . Chromatogr. A ,1998 , 817 : 337 —343
[41]	Xue Q , Yeung E S. Anal . Chem. , 1994 , 66 : 1175 —1178
[42]	Wang Z, Yeung E S. J . Chromatogr. B , 1997 , 695 : 59 —65
[43]	Hu S , Lee R , Zhang Z, et al . J . Chromatogr. B , 2001 , 752 :307 —310
[44]	Hu S , Zhang L , Cook L M, Dovichi N J . Electrophoresis , 2001 ,22 : 3677 —3682
[45]	Zhang H , Jin W R. Electrophoresis , 2004 , 25 : 1090 —1095
[46]	Schilling E A , Kamholz A E , Yager P. Anal . Chem. , 2002 , 74 :1798 —1804
[47]	Klepà rník K, Horky M. Electrophoresis , 2003 , 24 : 3778 —3783
[48]	Wu H , Wheeler A , Zare R N. Proc. Natl . Acad. Sci . USA ,2004 , 101 : 12809 —12813
[49]	Feltus A , Hentz N G, Daunert S. J . Chromatogr. A , 2001 , 918 :381 —392
[50]	Wang F , Tan W B , Zhang Y, et al . Nanotechnology , 2006 , 17 :R1 —R13
[51]	Wang Y L. Cell Biology —A Laboratory Handbook ( ed. Celis J E) . New York : Academic Press , 1994. 16 —21
[52]	Chakrabarti R and Schuster S M. Electroporation of antibodies intomammalian cells Cell Biology —A Laboratory Handbook (ed. Celis J E) . New York : Academic Press , 1994. 44 —49
[53]	Rosenthal S J , Tomlinson I , Adkins E M, et al . J . Am. Chem.Soc. , 2002 , 124 : 4586 —4594
[54]	Tully E , Hearty S , Leonard P , O’Kennedy R , Int . J . Bio.Mac. , 2006 , 39 : 127 —134
[55]	[1 ] McClain M A , Culbertson C T, Ramsey J M, et al . Anal .Chem. , 2003 , 75 : 5646 —5655
[56]	[2 ] Dovichi N J , Hu S. Current Opinion in Chemical Biology , 2003 ,7 : 603 —608
[57]	[3 ] Sims C E , Allbritton N L. Lab Chip , 2007 , 7 : 423 —440
[58]	[4 ] Kennedy R T, Oates M D , Copper B R , Jorgenson J W, et al .Science , 1989 , 246 : 57 —63
[59]	[6 ] Zhang H , Jin W R. Electrophoresis , 2004 , 25 : 480 —486
[60]	[7 ] Graessmann A , Graessmann M, Mueller C. Methods Enzymol . ,1980 , 65 : 817 —825
[61]	[8 ] Wick R , Angelova M I , Walde P , Luisi P L. Chem. Biol . ,1996 , 3 : 105 —111
[62]	Menger F M, Gabrielson K D , Angew. Chem. Int . Ed. Engl . ,1995 , 34 : 2091 —2106
[63]	Karlsson M, Nolkrantz K, Orwar O , et al . Anal . Chem. , 2000 ,72 : 5857 —5862
[64]	Gao J , Yin X F , Fang Z L. Lab Chip , 2004 , 4 : 47 —52
[65]	Ling Y Y, Yin X F , Fang Z L. Electrophoresis , 2005 , 26 :4759 —4766
[66]	Qin J , Ye N , Lin B , et al . Electrophoresis , 2005 , 26 : 1155 —1162
[67]	Song B , Wang G, Tan M, Yuan J . J . Am. Chem. Soc. , 2006 ,128 : 13442 —13450
[68]	Flors C , Fryer M J , Baker N R , et al . Journal of Experimental Botany , 2006 , 57 : 1725 —1734
[69]	Xu K, Tang B , Huang H , et al . Chem. Comm. , 2005 , 5974 —5976
[70]	Miller E W, Albers A E , Chang C J , et al . J . Am. Chem.Soc. , 2005 , 127 : 16652 —16659
[71]	Carter W, Narayanan P K, Robinson P. J . Leukoc. Biol . , 1994 ,55 : 253 —258
[72]	Kojima H , Urano Y, Kikuchi K. Angew. Chem. Int . Ed.Engl . , 1999 , 38 : 3209 —3212
[73]	Nolkrantz K, Farre C , Orwar O , et al . Anal . Chem. , 2002 , 74 :4300 —4305
[74]	Peterman M C , Noolandi J , Blumenkranz M S , Fishman H A.Proc. Natl . Acad. Sci . USA , 2006 , 101 : 9951 —9954
[75]	Zhang X, Yin H , Cooper J M, Haswell S J A. Eletrophoresis ,2006 , 27 : 5093 —5100
[76]	Inglefield J R , Schwartz-Bloom R D. Methods Enzymol . , 1999 ,307 : 469 —481
[77]	Griffin B A , Adams S R , Tsien R Y. Science , 1998 , 281 : 269 —272
[78]	Haugland R P. Handbook of Fluorescent Probes and Research Products (Molecular Probes) , 9th ed. Eugene , OR , USA , 2002
[79]	Fox M B , Esveld D C , Valero A , et al . Anal . Bioanal . Chem. ,2006 , 385 : 474 —485
[80]	Lundgvist J A , Sahlin F , Orwar O , et al . Proc. Natl . Acad. Sci .USA , 1998 , 95 : 10356 —10360
[81]	Ryttsen F , Farre C , Orwar O , et al . Biophys. J . , 2000 , 79 :1993 —2001
[82]	He H , Chang D C , Lee Y K. Bioelectrochemistry , 2007 , 70 :363 —368
[83]	Chen F G, Wang C , Zhi D Y, Xia G M. Amino Acids , 2005 ,29 : 235 —239
[84]	Moscho A , Orwar O , Zare R N , et al . Proc. Natl . Acad. Sci .USA , 1996 , 93 : 11443 —11447
[85]	Khalil A , Kogure K, Akita H , Harashima H. Pharmacol . Rev. ,2006 , 58 : 32 —45
[86]	Xiao H , Li X, Zou H , Yang L , et al . Anal . Chim. Acta , 2006 ,556 : 340 —346
[87]	Gilman S D , Ewing A G. Anal . Chem. , 1995 , 67 : 58 —64
[88]	Ocvirk G, Salimi-Moosavi H , Harrison A D , et al . Proc. IEEE ,2004 , 92 : 115 —125
[89]	Shi B , Huang W, Cheng J . Electrophoresis , 2007 , 28 : 1595 —1600
[90]	Craig D B , Dovichi N J . Anal . Chem. , 1998 , 70 : 2493 —2494
[91]	Parak W J , Pellegrino T, Plank C. Nanotechnology , 2005 , 16 :R9 —R25
[92]	Bruchez M J , Weiss S , Alivisatos A P , et al . Science , 1998 ,281 : 2013 —2016
[93]	Wu X, Liu H , Liu J , et al . Nat . Biotechnol . , 2003 , 21 : 41 —46
[94]	Pinaud F , King D , Moore H P , Weiss S. J . Am. Chem. Soc. ,2004 , 126 : 6115 —6123
[95]	Dahan M, Levi S , Triller A , et al . Science , 2003 , 302 : 442 —445
[96]	Lidke D S , Nagy P , Heintzmann R , et al . Nat . Biotechnol . ,2004 , 22 : 198 —203
[97]	Chu M, Song X, Cheng D , et al . Nanotechnology , 2006 , 17 :3268 —3273
[98]	Chen I , Choi YA , Ting A Y. J . Am. Chem. Soc. , 2007 , 129 :6619 —6625
[99]	Fan Z, Jin W. Talanta , 2007 , 72 : 1114 —1122

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133