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3,5-二甲基吡唑(DMP)施用后土壤硝化作用潜势及微生物群落动态变化研究

Keywords: 3,5-二甲基吡唑,硝化作用潜势,氨氧化细菌,反硝化细菌,微生物群落

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Abstract:

采用室内培养试验方法,从3,5-二甲基吡唑施用后土壤硝化作用潜势、参与氮转化的主要生理菌群、土壤微生物群落的数量及其生理活性等的动态变化入手,研究了新型硝化抑制剂3,5-二甲基吡唑(DMP)的施用对土壤硝化作用及生物学质量的影响。结果表明,适量DMP的施用可在一定时间内显著降低土壤的硝化作用潜势和土壤氨氧化菌的数量,刺激土壤三大微生物群落的增殖,但细菌、真菌和放线菌对DMP的剂量反应不同。土壤细菌数量呈现随DMP用量增加而增加的趋势,真菌和放线菌数量在培养28d之前则以中等剂量处理的为最高。超高量DMP的施用则强烈抑制土壤氨氧化菌的生长,土壤硝化作用潜势在整个培养期间一直维持在一个较稳定的低水平状态,且在施用前期对真菌和放线菌的增殖及总的微生物生理活性表现了一定的抑制效应,微生物商降低,qCO2值增大;在施用14d后,这种负效应逐渐缓解,进而表现出一定的刺激效应。DMP施用对土壤反硝化细菌影响不大。

References

[1]  李正名,陈寒松,赵卫光,等.吡唑衍生物的合成及生物活性[J].高等学校化学学报,1997,18(11):1794-1799.
[2]  Mary B,Recous S,Darwis D,et al.Interactions between decomposition of plant residues and nitrogen cycling in soil[J].Plant Soil,1996,181:71-82.
[3]  Kuzyakov Y,Domanski G.Carbon input by plants into the soil[J].J Plant Nutr Soil Sci,2000,163:421-431.
[4]  Scow K M.Soil microbial communities and carbon flow in agroecosystems[A].In:Jackson LE (ed.),Ecology in agriculture[M].Academic Press,San Diego,1997.367-413.
[5]  Insam H,Domsch K H.Relationship between soil organic carbon and microbial biomass on chrono-sequences of reclamation sites[J].Microbiol Ecol,1988,15:177-188.
[6]  Sparling G P.Ratio of microbial biomass carbon to soil organic C as a sensitive indicator of changes in soil organic matter[J].Aust J Soil Res,1992,30:195-207.
[7]  Patra A K,Chhonkar P K,Khan M A.Effect of green manure Sesbania sesban and nitrification inhibitor encapsulated calcium carbide (ECC)on soil mineral-N,enzyme activity and nitrifying organisms in a ricewheat cropping system[J].Europ J Soil Biol,2006,42:(in press).
[8]  Iizumi T,Mizumoto M,Nakamura K.A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors[J].Appl Environ Microbiol,1998,64:3656-3662.
[9]  Azam F,Benckiser G,Müller C,et al.Release,movement and recovery of 3,4-dimethylpyrazole phosphate (DMPP),ammonium,and nitrate from stabilized nitrogen fertilizer granules in a silty clay soil under laboratory conditions[J].Biol Fertil Soils,2001,34:118-125.
[10]  Macadam X M B,del Prado A,Merino P,et al.Dicyandiamide and 3,4-dimethylpyrazole phosphate decrease N2O emissions from grassland but dicyandiamide produces deleterious effects in clover[J].J Plant Physiol,2003,160:1517-1523.
[11]  Odum E P.Trends expressed in stressed ecosystems[J].Bioscience,1985,35:419-422.
[12]  Sakamoto K,Oba Y.Effect of fungal to bacterial biomass ratio on the relationship between CO2 evolution and total soil microbial biomass[J].Biol Fertil Soils,1994,17:39-44.
[13]  Quilchano C,Maranón T.Dehydrogenase activity in Mediterranean forest soils[J].Biol Fertil Soils,2002,35:102-107.
[14]  Visser S,Parkinson D.Soil biological criteria as indicators of soil quality:soil microorganisms[J].Am J Altern Agri,1992,7:33-37.
[15]  Pascual J A,Garcia C,Hernandez T,et al.Soil microbial activity as a biomarker of degradation and remediation process[J].Soil Biol Biochem,2000,32:1877-1883.
[16]  Lima J A,Nahas E,Gomes A C.Microbial populations and activities in sewage sludge and phosphate fertilizer-amended soil[J].Appl Soil Ecol,1996,4:75-82.
[17]  Monkiedje A,Ilori M O,Spiteller M.Soil quality changes resulting from the application of the fungicides mefenoxam and metalaxyl to a sandy loam soil[J].Soil Biol Biochem,2002,34:1939-1948.
[18]  Machulla G.Soil microbial indicators and their environmental significance[J].J Soils Sedi,2003,3:229.
[19]  孙志梅,梁文举,武志杰,等.新型杂环氮化合物DMP的硝化抑制效应初探[J].应用生态学报,2006,17(2):201-204.
[20]  McCarty G W,Bremner J M.Inhibition of nitrification in soil by heterocyclic nitrogen compounds[J].Biol Fertil Soils,1989,8:204-211.
[21]  Schinner F,Ohlinger R,Kandeler E,et al.Methods in Soil Biology[M].Spring-Verlag Berlin Heidelberg,Berlin,1996.12-95.
[22]  许光辉,郑洪元.土壤微生物分析方法手册[M].北京:农业出版社,1986.
[23]  Jenkinson D S,Brookes P C,Powlson D S.Measuring soil microbial biomass[J].Soil Biol Biochem,2004,36:5-7.
[24]  Belser L W.Population ecology of nitrifying bacteria[J].Annu Rev Microbiol,1979,33:309-313.
[25]  孙志梅,武志杰,陈利军,等.3,5-二甲基吡唑对尿素氮转化及硝酸盐淋溶的影响[J].环境科学,2007,28(1):(待刊).
[26]  Torsvik V,Goksoyr J,Daae F R.High diversity in DNA of soil bacteria[J].Appl Environ Microbiol,1990,56:782-787.
[27]  Haynes R J.Size and activity of the soil microbial biomass under grass and arable management[J].Biol Fertil Soils,1999,30:210-216.
[28]  任天志,Grego S.持续农业中的土壤生物指标研究[J].中国农业科学,2000,33(1):68-75.
[29]  Anderson I C,Poth M,Homstead J,et al.A comparison of NO and N2O production by the autotrophic nitrifier Nitrosomonas europaea and the heterotrophic nitrifier Alcaligenes faecalis[J].Appl Environ Microbiol,1993,59:3525-3533.
[30]  Brookes P C.The use of microbial parameters in monitoring soil pollution by heavy metals[J].Biol Fertil Soils,1995,19:269-279.
[31]  Avrahami S,Conrad R,Braker G.Effect of soil ammonium concentration on N2O release and on the community structure of ammonia oxidizers and denitrifiers[J].Appl Environ Microbiol,2002,68:5685-5692.
[32]  Mendum T A,Hirsch P R.Changes in the population structure of βgroup autotrophic ammonia oxidizing bacteria in arable soils in response to agricultural practice[J].Soil Biol Biochem,2002,34:1479-1485.
[33]  Machado J O,Nahas E,Sartori A L,et al.Variation of the population density of some microorganism in glucose treated latosoil[J].Cientifica,1980,8:77-88.
[34]  Chander K,Brookes P C.Microbial biomass dynamics during the decomposition of glucose and maize in metal-contaminated and non-contaminated soils[J].Soil Biol Biochem,1991,23:917-925.

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