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Cd~(2+)和Cu~(2+)对美人蕉的氧化胁迫及抗性机理研究

Keywords: 美人蕉,重金属,氧化胁迫,抗性机理,植物修复

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Abstract:

通过测定植物的丙二醛(MDA)含量、酸溶性SH含量以及两种抗氧化酶(超氧化物歧化酶SOD和愈创木酚过氧化物酶GPX)的酶活,研究了重金属镉(Cd)和铜(Cu)对美人蕉(CannaindicaLinn.)的氧化胁迫。结果表明,20μmol·L~(-1)和100μmol·L~(-1)的Cd~(2+)和Cu~(2+)均使其根部MDA含量显著增加,但除了100μmol·L~(-1)的Cd~(2+),叶部MDA含量无明显变化。与Cu~(2+)相比,Cd~(2+)能引起植物根部GSH的明显提高,并能诱导PCs的产生。Cd~(2+)使根部SOD活性明显增加,Cu~(2+)对其活性无明显影响。两种重金属均使其根部GPX活性显著增加。对于叶,重金属胁迫能增加GPX的活性,但对SOD活性的影响不明显。以上结果表明,重金属镉和铜对美人蕉具有氧化胁迫作用,而美人蕉具有缓解重金属胁迫的解毒机制,诱导PCs解毒,同时它能通过调节抗氧化物酶的活性增强其抗氧化胁迫的能力,这为该植物作为中低浓度镉和铜污染水体的修复植物种类应用提供了可能。

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