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利用3种in vitro 方法比较研究污染土壤中铅、砷生物可给性

Keywords: in,vitro,方法,,,生物可给性

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Abstract:

采用PBET(PhysiologicallyBasedExtractionTest)、SBET(SimpleBioavailabilityExtractionTest)和IVG(InVitroGastrointestinal)3种invitro方法研究采自浙江上虞的4种污染土壤中铅、砷的生物可给性。结果表明,3种方法得到的铅、砷在模拟胃肠液中的溶解度不同,胃阶段,采用SBET方法得到的数值最大,放有食物的IVG方法得到的数值最小;小肠阶段,不添加食物的IVG方法得到的数值最大,放有食物的IVG方法得到的数值最小。3种方法得到的铅、砷在模拟胃肠液中的生物可给性不同,胃阶段,采用SBET方法得到的数值最大,4种土壤中铅的生物可给性分别为89.2%、71.3%,56.2%和43.7%,砷的生物可给性分别为7.4%、6.6%,5.9%和7.4%。放有食物的IVG方法得到的数值最小,4种土壤中铅的生物可给性分别为30.1%、26.9%、18.4%和15.0%,砷的生物可给性分别为3.8%、4.9%、2.8%和3.4%。小肠阶段,不添加食物的IVG方法得到的数值最大,4种土壤中铅的生物可给性分别为13.9%、11.1%、21.8%和7.7%,砷的生物可给性分别为7.1%、7.3%、4.6%和7.3%。放有食物的IVG方法得到的数值最小,4种土壤中铅的生物可给性分别为3.4%、1.7%、4.3%和1.9%,砷的生物可给性分别为3.3%、4.8%、2.8%和2.8%。

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