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铜胁迫对两种不同来源长萼鸡眼草生长及氮代谢的影响

Keywords: 长萼鸡眼草,Cu胁迫,营养缺失,氮代谢,生长

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Abstract:

采用苗期水培的方法,研究铜胁迫和营养缺失及二者的复合作用对矿区和非矿区长萼鸡眼草生长及氮代谢的影响差异。结果表明,植物吸收的Cu绝大部分分配在植物的根部。Cu胁迫使植物组织中硝态氮(NO-3)含量和硝酸还原酶(NR)活性降低,使植物叶中铵态氮(NH+4)含量和谷氨酰胺合成酶(GS)活性升高,植物生物量减少。Cu胁迫下矿区植物叶中Cu含量、根中GS活性比非矿区的低,矿区植物叶生物量、组织中NR活性比非矿区的高。营养缺失使植物组织中NH+4含量、NO-3含量、NR活性、GS活性显著降低,根生物量显著增加,且矿区植物叶中GS活性的变化比非矿区的少。Cu胁迫营养缺失复合作用使植物组织中Cu含量、叶中GS活性显著升高,使根生物量、根中NR活性显著降低,且矿区植物叶中Cu含量增加的比非矿区的少。低营养胁迫可促进长萼鸡眼草对外源Cu的吸收,同时也刺激了植物根的生长,Cu胁迫和营养缺失环境下非矿区植物氮代谢的紊乱程度要比矿区的高,来源于矿区的植物对贫瘠和高Cu环境表现出更强的适应性。Cu及营养缺失对植物氮代谢的影响是非常复杂的,与植物组织中可利用氮和碳的改变有关,也与植物组织中酶活性的直接改变有关。

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