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富营养化浅水湖泊蓝藻的种群结构和多样性研究

Keywords: 浅水湖泊,富营养化,蓝藻种群结构,多样性,T-RFLP

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Abstract:

比较研究不同富营养化浅水湖泊中蓝藻种群结构和多样性,对于认识蓝藻水华的区域分布特征具有重要的生态和环境意义。于2009年7月在富营养化严重的滇池、巢湖和太湖各选取4个采样点,采用16S~23SrRNA基因间隔区(Internaltranscribedspacer,ITS)序列作为生物标记,通过末端限制性片段长度多态性技术(Terminalrestrictionfragmentlengthpolymorphism,T-RFLP)分析蓝藻种群结构和多样性。实验共得到36个不同的末端限制性酶切片段(Terminalrestrictionfragment,T-RF),反映3个湖泊中蓝藻种群具有丰富的基因多样性。T-RFLP聚类分析结果表明,在所研究的湖泊中蓝藻种群结构存在空间差异性,其中滇池和巢湖间蓝藻种群结构更为相似。环境因子与蓝藻种群的多元统计分析表明,正磷酸盐(PO4-P)和pH与附着细菌样品蓝藻种群结构动态变化具有显著的相关性(P<0.05),电导率(Conductivity)和总有机碳(TOC)与浮游细菌样品蓝藻种群结构动态变化具有显著的相关性(P<0.05)。因此,不同湖泊蓝藻水华的基因型和驱动因子存在差异。

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