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三种重金属对中华稻蝗金属硫蛋白基因表达的影响

DOI: 10.11654/jaes.2015.02.004

Keywords: 中华稻蝗 重金属 金属硫蛋白 精巢 卵巢 肌肉

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Abstract:

以栖息于稻田且主要取食水稻茎叶的中华稻蝗(Oxya chinensis)成虫为供试材料,经氯化镉、氯化铜和硫酸锌(CdCl2、CuCl2和ZnSO4)3种重金属急性染毒,采用RT-qPCR技术检测中华稻蝗2个金属硫蛋白基因(Oxya chinensis metallothionein,OcMT1, OcMT2)在精巢、卵巢和肌肉中的表达变化.结果表明,3种重金属均可诱导中华稻蝗MT基因不同程度的上调表达.经Cd急性处理后,3个器官组织中OcMT1和OcMT2表达水平在一定浓度范围内随着Cd浓度的增高而诱导表达上调;经Cu急性处理后,精巢和卵巢中OcMT1在最低浓度时的诱导表达量最高,OcMT2表达水平随着Cu浓度的增大而升高,肌肉中2个MT基因随着Cu浓度的增大而升高;经Zn处理后,OcMT1和OcMT2表达水平随着Zn浓度的升高而升高.由实验结果可以看出,3种重金属对中华稻蝗MT在不同器官组织均有诱导表达作用,但各浓度处理后其诱导表达水平不同,OcMT对不同重金属的敏感性具有显着差异

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