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海洋科学  2012 

劳盆地热液喷口沉积物微生物多样性初步研究

, PP. 23-27

Keywords: 劳盆地,热液喷口,沉积物,微生物多样性,聚合酶链式反应限制性片段长度多态性分析(PCR-RFLP)

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Abstract:

采用聚合酶链式反应限制性片段长度多态性分析(PCR-RFLP)技术对劳盆地热液区TVG9站位沉积物中细菌、古菌多样性进行了调查,并构建其细菌、古菌种群的16SrRNA基因文库。研究结果表明,该站位细菌包含变形杆菌(Proteobacteria)、硝化螺旋菌(Nitrospira),疣微菌(Verrucomicrobia)等3个类群,其中变形杆菌占据优势地位,由Alphaproteobactria,Gamaproteobactria,Deltaproteobactria等3个亚群组成。古菌包含泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),其中泉古菌占优势,由MGⅠ(MarineGroupⅠ)和MCG(MiscellaneousCrenarchaeotalGroup)两类群组成,而广古菌主要由MBGE(MarineBenthicGroupE)组成。旨在揭示深海热液区的微生物多样性,为生态环境的研究提供理论支持。

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