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石油污染土壤中芘高效降解菌群的筛选及降解特性研究

Keywords: 多环芳烃,,混合菌,生物降解

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Abstract:

从长期受石油污染土壤中驯化筛选到能以芘为惟一碳源生长的混合菌群GP3,其主要由假单胞菌株GP3A(Pseudomonassp.)和菌株GP3B(Pandoraeapnomenusa)组成。采用摇瓶振荡培养方法,研究了不同环境条件对混合菌GP3降解芘效能的影响。结果表明,在30℃,150r/min振荡培养下,混合菌GP3对15mg/L芘的7d降解率为90.6%。混合菌GP3降解芘的最适宜温度为35℃,最佳pH值为6.2。加入低浓度葡萄糖(100mg/L)或菲(10mg/L)作为共代谢底物,均可提高GP3对芘的降解率。混合菌对芘的降解速率(PDR)与芘的初始浓度呈正相关。研究重金属离子胁迫下GP3对芘的降解时发现,10mg/LZn2+的存在对芘降解效能影响较小,Cu2+对芘的降解有抑制作用,Cd2+对混合菌GP3有很强的毒性。

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