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高效阿特拉津降解菌株DNS10降解条件优化

Keywords: 阿特拉津(AT),菌株DNS10,降解能力,环境因素

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Abstract:

从长期施用阿特拉津的寒地黑土耕层(0~10cm)土壤中筛选到一株能以除草剂阿特拉津为氮源生长的降解菌株,结合16SrRNA序列分析结果,将该菌株命名为Arthrobactersp.DNS10。在接种量为108CFU/mL的条件下,菌株DNS10在24h内对100mg/L阿特拉津的降解率为99.41%。单因子实验结果表明,菌株DNS10适宜生长和降解的条件范围是:温度25~35℃,pH值5.0~8.0,培养液盐度0.1%~2%,对阿特拉津最大耐受浓度可达1200mg/L。正交实验法进一步表明,该菌株保持较好生长及降解能力的最优方案是温度30℃,pH值7.5,培养液盐度0.5%。影响其降解能力的环境因素的主次顺序依次是:温度>盐度>pH值。

References

[1]  Ren J., Jiang K. Impact of atrazine disposal on the water resources of the Yang River in Zhangjiakou area in China. B. Environ. Contam. Tox.,2002, 68 (6): 893-900
[2]  Struthers J.K., Jayachandran K., Moorman T.B. Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contaminated soil. Appl. Environ. Microbio., 1998, 64 (9): 3368-3375
[3]  Zhang Y., Jiang Z., Cao B., et al. Metabolic ability and gene characteristics of Arthrobacter sp. strain DNS10, the sole atrazine degrading strain in a consortium isolated from black soil. Inter. Bio. Bio.,2011, 65 (8): 1140-1144
[4]  Birgit Puschner, Robert H. Poppenga, Linda J. Lowenstine, et al. Assessment of melamine and cyanuric acid toxicity in cats. Vet. Diagn. Invest.,2007, 19 (6): 616-624
[5]  Sajiaphan K., Wackett L.P., Shapir N. Arthrobacter aurescens TC1 atrazine catabo lism genes trzN, a tzB and atzC are linked on a 160-kilobase region and are functional in Esch erichia coli. Appl. Environ. Microbio.,2004, 70 (7): 4402-4407
[6]  Nagy I., Compernolle F., Ghys K. J., et al. A single cytochrome P-450 system is involved in degradation of the herbicide EPTC (s-ethyldipropylthiocarbamate) and atrazine by Rhodococcus sp. strain NI86/21. Appl. Environ. Microbio.,1995, 61(5): 2056-2060
[7]  任登洲, 高小朋, 贺晓龙, 等. 阿特拉津降解菌的筛选及降解性能研究. 江苏农业科学,2009, (5):306-308 Ren Dengzhou, Gao Xiaopeng, He Xiaolong, et al. Study on the isolation and degradation performance of atrazine degradation bacteria. Agricultural Science of Jiangsu,2009, (5):306-308 (in Chinese)
[8]  郑柳柳, 袁 博, 朱希坤, 等. 阿特拉津降解菌株的分离、鉴定和工业废水生物处理试验. 微生物学通报,2009, 36 (7): 1099-1104 Zheng Liuliu, Yuan Bo, Zhu Xikun, et al. Isolation and characterization of atrazine-degrading strains and biotreatment experiment of industrial wastewater. Microbiology,2009, 36 (7): 1099-1104 (in Chinese)
[9]  薛锋, 高健. 苯酚降解菌的固定化及其降解特性的研究. 化学与生物工程,2010, 27(12): 72-75 Xue Feng, Gao Jian. Study on immobilization of phenol-degrading strain and its degradation performance.Chemistry & Bioengineering,2010, 27(12): 72-75 (in Chinese)
[10]  刘爱菊, 朱鲁生, 王军, 等. 除草剂阿特拉津的环境毒理研究进展. 土壤与环境,2002, 11(4): 405-408 Liu Aiju, Zhu Lusheng, Wang Jun, et al. Progress of study on the environmental toxicology of the herbicide atrazine. Soil and Environmental Sciences,2002, 11(4): 405-408 (in Chinese)
[11]  Vaishampayan P.A., Kanekar P. P., Dhakephalkar P. K. Isolation and characterization of Arthrobacter sp. strain MCM B-436, an Atrazine-degrading bacterium, from Rhizospheric soil. Inter. Bio. Bio.,2007, 60(4): 273-278
[12]  刘宏生, 姜薇, 宋雅娜, 等. 莠去津高效降解菌的筛选及鉴定. 辽宁工程技术大学学报(自然科学版),2009, 28 (1): 145-148 Liu Hongsheng, Jiang Wei, Song Yana, et al. Isolation and identification of atrazine degrading microbe. Journal of Liaoning Technical University(Natural Science),2009, 28 (1): 145-148 (in Chinese)
[13]  Kadian N., Gupta A., Satya S., et al. Biodegradation of herbicide (atrazine) in contaminated soil using various bio-processed materials. Bioresour. Technol.,2008, 99(11): 4642-4647
[14]  Liu C., Huang X., Wang H. Start-up of a membrane bioreactor bio-augmented with genetically engineered microorganism for enhanced treatment of atrazine containing wastewater. Desalination,2008, 231(1-3):12-19
[15]  Mahía J., Martín A., Díaz-Ravi?a M. Extractable atrazine and its metabolites in agricultural soils from the temperate humid zone. Environ. Geochem. Hlth.,2008, 30(2): 147-152
[16]  Senior E., Ralebisto T.K., Van Verseveld H.W. Microbial aspects of atrazine degradation in natural environments. Biodegradation,2002, 13(1):11-19
[17]  Jeannette A. Weiner, Marie E. DeLorenzo, Michael H. Fulton. Atrazine induced species-specific alterations in the subcellular content of microalgal cells. Pestic. Biochem. Phys.,2007, 87(1): 47-53
[18]  王辉, 周春燕, 李宝明, 等. 微生物降解阿特拉津的研究进展. 土壤通报,2005, 36 (5): 791-794 Wang Hui, Zhou Chunyan, Li Baoming, et al. Degradation of atrazine by microorganism:A review. Chinese Journal of Soil Science,2005, 36 (5): 791-794 (in Chinese)
[19]  刘娜, 杨云龙. 生物修复技术在污染环境修复中的应用研究. 科技情报开发与经济,2005, 15(3): 173-174 Liu Na, Yang Yunlong. Application study of bioremediational technique in polluted environment remediation. Sci./Tech. Information Development & Economy,2005, 15(3): 173-174 (in Chinese)
[20]  周宁, 王荣娟, 孟庆娟, 等.寒地黑土中阿特拉津降解菌的筛选及降解特性. 环境工程学报,2008, 2(11): 1560-1563 Zhou Ning, Wang Rongjuan, Meng Qingjuan, et al. Screening and identification of atrazine degrading bacteria and their degradation characteristics from black soil in cold area. Chinese Journal of Environmental Engineering,2008, 2(11): 1560-1563 (in Chinese)
[21]  李宝明, 孙军德, 王辉等. 阿特拉津降解细菌的筛选和鉴定. 土壤通报,2007, 8 (3): 619-621 Li Baoming, Sun Junde, Wang Hui, et al. Isolation and screening of atrazine degradation bacteria. Chinese Journal of Soil Science,2007, 8 (3): 619-621 (in Chinese)

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