全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

固态发酵法制备优势硝化菌固定化产品的工艺优化

Keywords: 微生物固定化,固态发酵,TTC-脱氢酶活性,响应面法,焦化废水

Full-Text   Cite this paper   Add to My Lib

Abstract:

为解决高效菌在工程应用中扩大培养、保存和接种等瓶颈,研究固态发酵法制备焦化废水优势硝化菌固定化产品。以TTC脱氢酶活性法作为固体发酵生物量测定指标,通过液态发酵单因素实验初步确定硝化菌发酵条件,再用响应面法对其固态发酵条件进行优化。结果表明,TTC脱氢酶活性法适合作为表征生物质载体固定化产品生物量的测定方法,不受载体和培养时间的影响,能较好地指示干燥前固态发酵生物量的变化。固态发酵主要影响参数依次为发酵时间、发酵温度、接种量和含水率。发酵时间的最佳值为47h,发酵温度最佳为29.4℃,接种量的最佳值为9.9mL/200mL,最佳含水率为48.9%时,固态发酵固定化产品酶活达到17.091μgTF/(g·h)。与液态发酵参数相比其发酵温度、接种量最佳值接近,但发酵时间由1d延长至2d,产品酶活持平。

References

[1]  王建芳,赵庆良,林佶侃,等.生物强化技术及其在废水生物处理中的应用.环境工程学报,2007,1(9):40-45 Wang Jianfang,Zhao Qingliang,Lin Jikan,et al. Bioaugmentation technology and its application inbiological wastewater treatment. Chinese Journal of Environmental Engineering,2007,1(9):40-45(in Chinese)
[2]  徐军祥, 杨翔华, 姚秀清,等. 生物强化技术处理难降解有机污染物的研究进展.化工环保, 2007, 27(2):129-134 Xu Junxiang, Yang Xianghua1,Yao Xiuqing, et al. Advances in bioaugmentation technology for treatment of refractory organic pollutants. Environmental Protection of Chemical Industry, 2007, 27(2):129-134 (in Chinese)
[3]  梅琴,高国庆,虞方伯,等. 对硝基苯酚冲击对UASB反应器中污泥活性和微生物种群的影响. 微生物学通报, 2007,34(6):1123-1128 Mei Qin,Gao Guoqing,Yu Fangbo, et al. Effect of p-nitrophenol shock on sludge activity and microbial populations and in UA SBR eactor.Microbiology, 2007,34(6):1123-1128(in Chinese)
[4]  韦朝海,贺明和,任源,等.焦化废水污染特征及其控制过程与策略分析.环境科学学报, 2007, 27(7): 1083-1093 Wei C. H., He M. H., Ren Y., et al. Pollution characteristics of coking wastewater and control strategies: Biological treatment process and technology. Acta Scientiae Circumstantiae, 2007, 27(7): 1083-1093(in Chinese)
[5]  蔡昌凤,孙菲.一种微生物的固定化方法. 200910251725.7 Cai Changfeng, Sun Fei. A methods of microorganism immobilized.CN200910251725.7(in Chinese)
[6]  许赣荣,胡文锋.固态发酵原理、设备与应用.北京:化学工业出版社,2009
[7]  Reeta Rani Singhania, Rajeev K. Sukumaran, Anil Kumar Patel. Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme and Microbial Technology, 2010, 46(7): 541-549
[8]  魏培莲,岑沛霖,盛春琦.3种固态发酵生物量测定方法的比较. 食品与生物技术学报,2006,25(1):60-64 Wei Peilian, Cen Peilin, Sheng Chunqi. Comparison of three biomass estimation methods in solid state fermentation. Journal of Food Science and Biotechnolog,2006,25(1):60-64(in Chinese)
[9]  朱小彪,许春华,高宝玉,等.曝气生物滤池生物量和生物活性的试验研究.环境科学学报, 2007,27(7): 1135-1140 Zhu Xiaobiao, Xu Chunhua, Gao Baoyu, et al. Studies of biomass and m icrobial activity in a biological aerated filter. Acta Scientiae Circumstantiae, 2007,27(7): 1135-1140(in Chinese)
[10]  胡子斌. TTC—脱氢酶活性常温萃取测定法及应用.工业水处理, 2001,21(10):29-31 Hu Zibin. Normal temperature extraction for the determination of TTC-dehydrogenase activity and its application. Industrial Water Treatment, 2001,21(10):29-31(in Chinese)
[11]  李今,吴振斌,贺锋. 生物膜活性测定中TTC-脱氢酶活性测定法的改进. 吉首大学学报(自然科学版), 2005,26(1):37-39 Li Jin,Wu Zhenbin,He Feng. Improvement of TTC-dehydrogenase activity determination method in biological activity determination. Journal of Jishou University (Natural Science), 2005,26(1):37-39(in Chinese)
[12]  秦俊哲,李亚杰,帅斌. 响应面法优化桑黄液体发酵培养条件的研究. 食品科技, 2010,35(11):7-10 Qin Junzhe,Li Yajie,Shuai Bin. Optimization of liquid fermentation conditions for phellinus linteus using response surface methodology. Food Science and Technology, 2010, 35(11):7-10(in Chinese)
[13]  Hasan Fariha, Shah AamerAli, Hameed Abdul. Industrial applications of microbial lipases. Enzyme and Microbial Technology, 2006,39(2):235-251
[14]  张晖,Huang Chin-Pao. 响应面法分析卫生填埋渗滤液的Fenton处理过程. 环境科学, 2003,24(4):140-143 Zhang Hui, Huang Chin-Pao. Evaluation of Fenton process for the treatment of sanitary landfill leachate with response surface methodology. Environmental Science, 2003, 24(4):140-143(in Chinese)
[15]  王海晶,王玉华,齐斌. 响应面法优化产丙酸杆菌素菌株的培养条件. 中国乳品工业,2011,39(2):13-16 Wang Haijing,Wang Yuhua,Qi Bin. Optimization of propionin production by Response Surface Method. China Dairy Industry, 2011,39(2):13-16(in Chinese)
[16]  李家元,吴彦瑜,周少奇. 响应曲面法优化絮凝处理木薯淀粉废水. 环境工程学报, 2010,4(7):1555-1560 Li Jiayuan, Wu Yanyu, Zhou Shaoqi. Application of response surface methodology (RSM) to optimize coagulation treatment of cassava starch wastewater. Chinese Journal of Environmental Engineering, 2010, 4(7):1555-1560(in Chinese)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133