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石油降解菌的细胞表面疏水特性研究

Keywords: 石油菌,培养基,环境因子,疏水性

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Abstract:

实验采用从受污染水体分离出的6株石油降解菌D3、T4、R4、T1、D4和R3,对石油菌细胞表面疏水性的测定方法进行了研究并分析了环境因子对细胞表面疏水性的影响。结果表明,在采用葡萄糖培养基测定的细胞表面疏水率误差最小,最优的菌悬液与二甲苯的比值为4:1.5;细胞表面疏水率在高盐度条件下会下降,初始pH在中性环境时数值最高,温度在25℃时疏水性最好。对比表面疏水率与柴油降解率随时间的变化得出细胞表面疏水性与柴油降解率具有一定的相关性,细胞表面疏水率在培养3d之后基本趋于稳定。

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