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葵花浓缩蛋白的酶法改性研究

, PP. 86-90

Keywords: 葵花籽,浓缩蛋白,酶水解,电泳,功能性

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Abstract:

采用1.398枯草杆菌中性蛋白酶对葵花浓缩蛋白进行水解,降解蛋白分子,通过电泳分析了不同水解度蛋白亚基的变化,系统地分析了不同水解度葵花酶解蛋白与葵花浓缩蛋白的功能特性。实验表明,水解度6.5%的葵花酶解蛋白溶解性、吸油性、乳化性等功能性得到较大的改善,而葵花浓缩蛋白持水性、起泡性及起泡稳定性好于葵花酶解蛋白,葵花浓缩蛋白经酶解后游离的氨基酸含量都有明显提高。葵花蛋白除赖氨酸含量稍低外,其他必需氨基酸组成与NAS(1980)和FAO(1973)参考模式相比,具有良好平衡性。

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