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科技导报  2014 

粉红粘帚霉ACM941固体发酵条件的优化

DOI: 10.3981/j.issn.1000-7857.2014.12.003, PP. 26-30

Keywords: 粉红粘帚霉,孢子浓度,固体发酵培养基,发酵条件

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Abstract:

为提高粉红粘帚霉ACM941发酵过程中的分生孢子产量,本研究对其固体发酵条件进行了优化。以产孢量为测定指标,通过单因子和正交试验,确定ACM941固体发酵基础培养基为麦麸和棉籽壳混合物,其比例为46;在基础培养基中添加2%玉米粉、2%燕麦粉、3%牛肉膏、2%蛋白胨可获得更大产孢量;对发酵温度、接种量等发酵条件进行了优化,确定发酵培养基初始含水量为30%;种龄48h;接种量6%;发酵过程中24h全光照;菌丝生长温度25℃,产孢温度20℃。在最佳发酵条件下,ACM91产孢量达1.17×109cfu/g培养物,比初始发酵水平提高了12.4倍,为生防制剂的开发奠定了基础。

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