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油菜根系质膜H+-ATPase活性对缺磷胁迫的反应

DOI: 10.7685/j.issn.1000-2030.2008.02.015, PP. 71-75

Keywords: 油菜,缺磷,细胞膜H+-ATPase,两相法

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Abstract:

采用两相法分离水培油菜根系细胞膜,并测定了细胞膜H+-ATPase水解活性。结果表明,与供磷植物相比,活体条件下,缺磷处理植株根系大量分泌H+并被钒酸盐抑制,缺磷处理植株磷含量明显下降。细胞膜纯度达90%以上。离体条件下,缺磷和对照植株细胞膜H+-ATPase的最佳pH值均为6.0左右;缺磷油菜根系细胞质膜上H+-ATPase水解活性和Vmax略有升高,Km和对钒酸盐的敏感性则降低。表明油菜根系细胞膜H+-ATPase水解活性升高是其适应缺磷的一种生理机制,通过提高H+-ATPase的活性很可能促进有机酸的分泌,从而活化根际土壤中被固定的磷。

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