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茶树ATP硫化酶和硒半胱氨酸甲基转移酶基因植物表达载体的构建

DOI: 10.7685/j.issn.1000-2030.2008.02.025, PP. 121-125

Keywords: 茶树,ATP硫化酶,硒半胱氨酸甲基转移酶,基因,植物表达载体

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Abstract:

采用能有效转化双子叶植物的表达载体pBI121构建植物硒营养代谢关键酶基因的表达载体,将原载体中GUS基因用茶树ATP硫化酶基因(APS1和APS2)替换,将硒半胱氨酸甲基转移酶基因(CsSMT)连接到pBI121载体上直接与GUS基因相连,分别构建了目的基因植物表达载体pBI-APS1、pBI-APS2和pBI-CsSMT。通过三亲杂交法将重组质粒导入根癌农杆菌LBA4404中,获得转化工程菌,为通过植物基因工程获得富硒农产品打下基础。

References

[1]  萨姆布鲁克?J,拉塞尔 D W,黄培堂.分子克隆实验指南[M].北京:科学出版社.2002
[2]  王朝霞.茶树巯基蛋白酶抑制剂基因的克隆及表达分析[D].合肥:安徽农业大学.2006
[3]  Pilon-Smits E A H,Hwang S,Lytle C M.Overexpression of ATP sulfurylase in Indian mustard leads to increased selenate uptake,reduction,and tolerance[J].Plant Physiology.1999,119:123-132
[4]  Terry N,Zayed A M,de Souza M P.Selenium in higher plants[J].Annual Review of Plant Physiology and Plant Molecular Biology.2000,51:401-432
[5]  Neuhierl B,Thanbichler M,Lottspeich F.A family of S-methylmethionine-dependent thiol/selenol methyltransferases:role in selenium tolerance and evolutionary relation[J].Journal of Biological Chemistry.1999,274:5407-5414
[6]  Zhu L,Jiang C J,Deng W W.Cloning and expression of selenocysteine methyltransferase cDNA from Camellia sinensis[J].Acta Physiologiae Plantarum.2008,30:167-174
[7]  Pickering I J,Wright C,Bubner B.Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus[J].Plant Physiology.2003,131:1460-1467
[8]  王关林,方宏筠.植物基因工程[M].北京:科学出版社.2002
[9]  Neuhierl B,B(o)ck A.On the mechanism of selenium tolerance in selenium-accumulating plants purification and characterization of a specific selenocysteine methyltransferase from cultured cells of Astragalus bisculatus[J].European Journal of Biochemistry.1996,239:235-238
[10]  Lyi S M,Heller L I,Rutzke M.Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli[J].Plant Physiology.2005,138:409-420
[11]  LeDuc D L,Tarun A S,Montes-Bayon M.Overexpression of selenocysteine methyltransferase in Arabidopsis and Indian mustard increases selenium tolerance and accumulation[J].Plant Physiology.2004,135:377-383
[12]  Zhu L,Deng W W,Ye A H.Cloning of two cDNAs encoding a family of ATP sulfurylase from Camellia sinensis related to selenium or sulfur metabolism and functional expression in Escherichia coli[J].Plant Physiology and Biochemistry.2007

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