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一种改良鸡的鸡胚原代肝细胞的分离培养方法

DOI: 10.7685/j.issn.1000-2030.2008.04.029, PP. 154-156

Keywords: 鸡胚肝细胞,原代培养,形态,功能

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Abstract:

剪切9日龄三黄肉鸡鸡胚肝组织,胰酶消化分离肝细胞,用改良的M199培养液进行培养。结果显示,分离的肝细胞即时存活率为93.7%±2.6%;过碘酸席夫试剂染色后,胞质中布满粉红色糖原颗粒;肝细胞活力和白蛋白分泌功能在第3天达到最高水平,这是体外研究禽类肝脏功能和代谢的最佳时机。

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