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温度预处理对不结球白菜杂种F1花药培养的影响

DOI: 10.7685/j.issn.1000-2030.2008.04.027, PP. 144-148

Keywords: 不结球白菜,花药培养,预处理

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Abstract:

以不结球白菜杂种一代‘99H秋-25’ב99H秋-23’和‘苏州青’בSI’为试材,研究了低温预处理和高温预培养对花药培养效果的影响。结果表明0~4℃的低温预处理花药1d,花粉可以完成其进行雄核发育所需要的诱导过程,而且绝大部分花粉(93.6%)仍然保持存活,花药分化率达到1.33%;而未经预处理的花药在诱导过程完成时,大多数退化死亡。高温(33℃,24h)处理可明显提高花药再生植株的诱导率。

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