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组织块种植法体外培养奶牛乳腺上皮细胞

DOI: 10.7685/j.issn.1000-2030.2008.04.017, PP. 91-96

Keywords: 奶牛,乳腺上皮细胞,体外培养,细胞形态,酪蛋白

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Abstract:

为建立成熟的奶牛乳腺上皮细胞体外培养体系,以组织块种植法培养荷斯坦泌乳期成年母牛乳腺组织,观测长出的上皮细胞在各生长时期的形态变化,绘制生长曲线并计算群体倍增时间,用免疫组化技术鉴定乳腺上皮细胞角蛋白18的表达,用SDS-PAGE电泳和免疫印迹分析技术检测乳腺细胞的酪蛋白分泌情况。试验结果表明,此培养体系可获得良好的奶牛乳腺上皮细胞,原代以及传代培养,细胞增殖旺盛,长势良好;细胞骨架蛋白――角蛋白18表达为阳性,且培养的细胞具有分泌牛乳酪蛋白的功能。

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