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宁夏稻瘟病菌群体遗传结构研究

DOI: 10.7685/j.issn.1000-2030.2008.04.012, PP. 66-72

Keywords: 稻瘟病菌,宁夏,遗传结构,遗传多样性,rep-PCR,DNA指纹

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Abstract:

采用Pot2-rep-PCR指纹识别技术对宁夏稻瘟病菌的群体结构进行分析。根据每个菌株的指纹型将2002至2005年在9个地区采集的406个稻瘟病菌单孢菌株鉴定为65个单元型,其中,单元型NXH7包含176个菌株,占总菌株数的43.3%,在所有年份、地区和大多数水稻品种上出现,是宁夏稻瘟病菌中最广泛适应的单元型。对稻瘟病菌群体的时空变化分析发现,不同年份、不同地区的稻瘟病菌亚群体间遗传分化不明显(地区间Gst=0.0832,年份间Gst=0.0509),遗传结构相似;不同水稻品种上的亚群体遗传分化明显(Gst=0.2823)。提示寄主的选择作用是宁夏稻瘟病菌群体演化的主要力量。宁夏稻瘟病菌菌株间有较高的相似性,总体相似性大于62%,DNA指纹没有任何清晰的系谱结构,没有任何聚类组被大于60%的bootstrap值支持。提示宁夏稻瘟病菌群体可能是单一的无性系谱占绝对优势。总体而言,宁夏稻瘟病菌群体遗传多样性水平较低(H=0.0963,I=0.1806)。

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