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江苏水稻品种抗稻瘟病遗传多样性研究

DOI: 10.7685/j.issn.1000-2030.2008.03.015, PP. 77-80

Keywords: 水稻,抗病基因同源序列(RGA),抗病表型,遗传多样性

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Abstract:

根据已知的不同抗病基因保守区域设计引物,通过在水稻基因组DNA中扩增和分离有相似序列的DNA片段,可以快速鉴定出候选抗病基因。选择3对扩增条带多样性较高的引物XLRRfor/XLRRrev、S1/AS3和Ptokin1/Ptokin2,对江苏27个水稻品种进行抗病基因同源序列PCR分析(resistancegeneanalog-PCR,RGA-PCR)。结果显示,3对引物共扩增出清晰可辨的谱带127条,其中多态性带101条,占总数的79.53%。根据聚类分析,3对引物对或引物组合扩增的RGA图谱可将品种分为多种类型,这些RGA图谱类型与稻瘟病菌7个生理小种接种鉴定的抗病表型没有完全的对应关系。

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