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利用mRNA差异显示技术克隆恶疫霉侵染诱导表达的草莓基因

DOI: 10.7685/j.issn.1000-2030.2012.04.010, PP. 54-58

Keywords: 草莓冠腐病,恶疫霉,mRNA差异显示技术

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Abstract:

为了解草莓与恶疫霉互作的分子机制,以接种后3、5和7d的感病草莓品种‘FDP821’的冠部为材料,利用mRNA差异显示技术研究‘FDP821’与恶疫霉亲和互作前后基因表达的差异,获得78条差异表达的cDNA片段,通过分析从中分离出恶疫霉侵染诱导表达的10个草莓基因cDNA片段。BLASTX在线分析表明,这些草莓基因的功能涉及呼吸作用、衰老和核苷运输与代谢等。为阐明草莓与恶疫霉互作的分子机制提供了重要的线索。

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