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菊花近缘种属植物抗蚜性机制研究

DOI: 10.7685/j.issn.1000-2030.2012.03.005, PP. 25-30

Keywords: 菊花,抗蚜性,叶片解剖结构,酶活性,基因表达

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Abstract:

以高抗蚜虫材料‘黄金艾蒿’和不抗材料菊花脑为材料,比较分析两者叶片解剖结构及蚜虫接种对防御酶活性和基因表达量变化的影响。结果表明高抗材料‘黄金艾蒿’栅栏组织厚度(77.3μm)、栅栏组织/海绵组织厚度比(0.9)大于不抗材料菊花脑(57.3μm、0.4),而海绵组织厚度(93.6μm)和层数(2.9层)小于菊花脑(139.8μm、6.2层)。接种蚜虫后,2种材料叶片过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)3种抗虫相关酶的活性及相关基因的表达量均有所提高,且高抗材料的上升速度比不抗材料快、幅度大,并在接种后期保持较高水平;2种材料的酶活性变化均比相对应的基因表达量变化滞后。抗感材料叶片解剖结构和蚜虫接种前后防御反应与抗虫水平相关,可以作为其苗期蚜虫抗性评价的指标。

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