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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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新型纳米纤维固相萃取柱制备及其用于组织样品中柔红霉素测定研究
, PP. 312-316
Keywords: 柔红霉素,纳米纤维,高效液相色谱
Abstract:
研制出一种新型纳米纤维固相萃取柱,并将其用于大鼠心、肝、脾、肺、肾组织及血浆中柔红霉素的净化、浓缩处理,建立了生物样品中柔红霉素的高效液相荧光检测法。以10%(V/V)HClO4为溶剂,对组织样进行匀浆和离心处理,上清液用纳米纤维小柱净化富集,以50mL含1%(V/V)冰醋酸的甲醇洗脱,进样20mL检测。流动相为甲醇-水-冰醋酸(60:40:0.25,V/V,pH5.5),流速为1.0mL/min。在5种组织中,方法的定量线性范围为0.02～20mg/g,在血浆中为0.01～1mg/L。方法的回收率为82.7%～112.3%,日内RSD为2.0%～9.9%,日间RSD为5.2%～11.9%。本方法具有快速、灵敏等优点,可应用于动物的各组织样中柔红霉素检测。
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