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电化学  2012 

纳米金放大计时库仑法的PML/RARα融合基因检测

, PP. 286-290

Keywords: 纳米金,计时库仑法,氯化六氨合钌,PML/RAR&alpha,融合基因

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Abstract:

应用恒电位在金基底表面电化学沉积纳米金,通过Au—S键将巯基修饰DNA探针固定在纳米金表面,并与互补靶序列杂交,构建计时库仑电化学DNA传感器,并检测急性早幼粒细胞白血病(APL)PML/RARα融合基因.采用电子扫描显微镜(SEM)与电化学交流阻抗技术(EIS)观察纳米金和表征DNA传感器的构筑过程.以氯化六氨合钌([Ru(NH3)6]Cl3,RuHex)作电化学杂交指示剂,由计时库仑法检测人工合成APL的PML/RARα融合基因.结果表明,纳米金能放大RuHex检测信号,杂交前后电量差值(?Q)与靶标链DNA浓度的对数(lgC)在1.0×10-13mol?L-1~1.0×10-9mol?L-1范围内呈线性关系,检出下限3.7×10-14mol?L-1(S/N=3).该法操作简便、特异性好,有望用于实际样品的检测.

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