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二氟甲基鸟氨酸对T淋巴瘤Jurkat细胞生长的影响

, PP. 593-596

Keywords: 二氟甲基鸟氨酸,T淋巴细胞,Jurkat细胞,细胞凋亡,生长抑制物

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Abstract:

目的研究二氟甲基鸟氨酸(difluromethylornithine,DFMO)对人T淋巴瘤Jurkat细胞的影响及其作用机制,为探讨DFMO能否用于治疗人白血病提供实验依据。方法DFMO(0~10mmol/L)处理人T淋巴瘤Jurkat细胞24~72h后,MTS法检测细胞的存活率,化学分析法检测精胺氧化酶(spermineoxidase,SMO)和乙酰多胺氧化酶(acetylpolyamineoxidase,APAO)活性,DNA片段化分析检测细胞凋亡,荧光染料法检测细胞线粒体膜电位变化,Westernblot法检测细胞内Bax含量,分光光度法检测caspase?3酶活性。结果DFMO处理细胞能显著抑制Jurkat细胞的生长(P<0.01),抑制率随药物浓度的增加和作用时间的延长而加大,DFMO浓度为10mmol/L时,24h抑制率为33%,48h抑制率为38%,72h抑制率为49%。DFMO处理可导致Jurkat细胞DNA片段化,促进Bax由细胞质向线粒体转移,细胞内caspase-3活性增加(增加46%,P<0.05),并伴有线粒体膜电位的显著降低,DFMO浓液为2和5mmol/L时,分别降低29%和53%(P<0.01)。DFMO还能诱导细胞内SMO活性(增加1.1倍,P<0.05)但对APAO的活性无诱导性影响。结论DFMO对人T淋巴瘤Jurkat细胞具有生长抑制和诱导凋亡的作用。

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