全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

锁阳多糖对去卵巢大鼠骨质疏松的改善作用

, PP. 2360-2363

Keywords: 锁阳多糖,骨质疏松,去卵巢大鼠,BGP,BMD,OPG/RANKL

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的观察锁阳多糖对去卵巢大鼠骨质疏松的作用并探讨其机制。方法雌性SD大鼠分为假手术组、造模组。术后2个月造模组分为模型组、阳性对照组、锁阳多糖治疗组,每组10只。治疗组灌胃给予锁阳多糖(20、40、80mg/kg),阳性药组灌胃给予尼尔雌醇(1.5mg/kg),每日1次,连续给药12周。ELISA法检测血清雌二醇(E2)、骨钙素(BGP)、降钙素(CT)及尿液脱氧吡啶啉(DPD)的含量,双能X线骨密度检测仪检测腰椎及股骨的骨矿物密度(BMD),三点弯曲法测定胫骨生物力学,Westernblot检测大鼠股骨组织中骨保护素(OPG)及核因子-κB受体活化因子配体(RANKL)蛋白的表达。结果摘除卵巢后模型组大鼠血清中E2、CT显著下降(P<0.05,P<0.01),血清BGP及尿液DPD含量则显著升高(P<0.01),中、高剂量锁阳多糖及尼尔雌醇能够显著升高血清E2、CT含量(P<0.05),降低血清BGP及尿液DPD含量(P<0.01);锁阳多糖及尼尔雌醇能够逆转去卵巢大鼠腰椎和股骨BMD的降低(P<0.01),改善胫骨生物学特性中的最大载荷及最大应力降低的趋势(P<0.05,P<0.01),并能增强OPG蛋白表达(P<0.05,P<0.01),降低RANKL蛋白表达(P<0.01),升高OPG/RANKL比值(P<0.01)。结论锁阳多糖有明显抗去卵巢大鼠骨质疏松的作用,其机制可能与其调节E2,CT、BGP和DPD水平,激活OPG/RANK/RANKL信号系统有关。

References

[1]  Chlebowski R T, Anderson G L, Gass M, et al. Estrogen plus progestin and breast cancer incidence and mortality in postmenopausal women[J]. JAMA, 2010, 304(15): 1684-1692. [2]于舒雁, 刘会丽, 苗明三. 锁阳的现代研究及展望[J]. 中医学报, 2012, 27(1): 79-81. [3]王晓梅, 张倩, 热娜, 等. 锁阳全草化学成分的研究[J]. 中草药, 2011, 42(3): 458-460. [4]马丽杰. 锁阳多糖延缓衰老的作用及端粒酶调控机制研究[D]. 呼和浩特: 内蒙古大学, 2009. [5]Terauchi M. Role of the immune system in the pathophysiology of postmenopausal osteoporosis[J]. Nihon Rinsho, 2011, 69(7): 1215-1219. [6]Rachner T D, Khosla S, Hofbauer L C. Osteoporosis: now and the future[J]. Lancet, 2011, 377(9773): 1276-1287. [7]Rossini M, Di-Munno O, Gatti D, et al. Optimising bisphosphonate treatment outcomes in postmenopausal osteoporosis: review and Italian experience[J]. Clin Exp Rheumatol, 2011, 29(4): 728-735. [8]Liang H D, Yu F, Tong Z H, et al. Cistanches Herba aqueous extract affecting serum BGP and TRAP and bone marrow Smad1 mRNA, Smad5 mRNA, TGF-β1 mRNA and TIEG1 mRNA expression levels in osteoporosis disease[J]. Mol Biol Rep, 2013, 40(2): 757-763. [9]Xu H, Xiao W, Luo D, et al. Association analysis of genetic polymorphisms and potential interaction of the osteocalcin (BGP) and ER-alpha genes with body mass index (BMI) in premenopausal Chinese women[J]. Acta Pharmacol Sin, 2010, 31(4): 455-460. [10]Stovall D W, Pinkerton J V. Estrogen agonists/antagonists in combination with estrogen for prevention and treatment of menopause-associated signs and symptoms[J]. Womens Health (Lond Engl), 2008, 4(3): 257-268. [11]Cheng Z, Yao W, Zimmermann E A, et al. Prolonged treatments with antiresorptive agents and PTH have different effects on bone strength and the degree of mineralization in old estrogen-deficient osteoporotic rats[J]. J Bone Miner Res, 2009, 24(2): 209-220. [12]Wasilewska A, Rybi-Szuminska A A, Zoch-Zwierz W. Serum osteoprotegrin (OPG) and receptor activator of nuclear factor kappaB (RANKL) in healthy children and adolescents[J]. J Pediatr Endocrinol Metab, 2009, 22(12): 1099-1104.
[2]  刘瑶瑶,代飞,孙东,等.不同量骨水泥强化新型空心椎弓根螺钉的体外生物力学研究[J].第三军医大学学报,2012,34(16):1626.  Liu Yaoyao,Dai Fei,Sun Dong,et al.Influence of the volume of bone cement on the reinforcement of a novel canulated pedicle screw: a biomechanical study in vitro[J].J Third Mil Med Univ,2012,34(23):1626. [2]陈秀明,艾国平,粟永萍,等.新型异黄酮类化合物对去势大鼠股骨生物力学性能影响的实验研究[J].第三军医大学学报,2007,29(11):1039.  CHEN Xiu-ming,AI Guo-ping,SU Yong-ping,et al.Effect of a novel isoflavone compound on femoral biomechanics of ovarectomied rats[J].J Third Mil Med Univ,2007,29(23):1039. [3]向菲,李树法,王培珊,等.贵阳市1 171例女性桡骨超声速率的研究[J].第三军医大学学报,2006,28(15):1627. [4]尹雅江.溃疡性结肠炎并多种肠外表现1例[J].第三军医大学学报,2013,35(06):546. [5]戈朝晖,丁惠强,梁思敏,等.单侧椎弓根外入路椎体后凸成形术治疗重度骨质疏松性椎体骨折[J].第三军医大学学报,2011,33(17):1868.  Ge Zhaohui,Ding Huiqiang,Liang Simin,et al.Percutaneous kyphoplasty through unilateral extrapedicular approach in treating severe osteoporotic vertebral compression fractures[J].J Third Mil Med Univ,2011,33(23):1868. [6]平少华,张岩,梁春雨.股骨近端抗旋转髓内钉与经皮微创锁定钛板治疗老年股骨粗隆间骨折的对比研究[J].第三军医大学学报,2014,36(06):621. [7]覃佳强,徐培菊,张德文,等.维生素K防治儿童肾病综合征继发性骨质疏松作用的研究[J].第三军医大学学报,2006,28(18):1891.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133