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电离辐射对小鼠肠道隐窝素4表达的影响及意义

, PP. 330-334

Keywords: 电离辐射,隐窝素,抗菌,LPS,IL-&beta

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Abstract:

目的检测小鼠受电离辐射后隐窝素4(cryptdin4,Crp4)的表达变化,探讨其在电离辐射肠损伤中的生物学意义。方法8周龄BALB/c小鼠采用随机数字表法分为两组,未辐照组(n=5)和辐照组(n=15),辐照组给予8GyX射线全身一次性照射,分别于照后9、24、72h取小鼠小肠组织和眼球血。免疫组织化学染色和Westernblot分析小鼠小肠Paneth细胞隐窝素Crp4的表达变化,鲎试剂法检测小鼠血清LPS水平。化学合成Crp4多肽,采用涂布克隆计数法分析Crp4对肠道条件致病菌(大肠杆菌、粪肠球菌)和益生菌(乳酸杆菌、双歧杆菌)的抗菌活性;同时,观察Crp4对LPS诱导促炎因子IL-1β生成的影响。结果与对照组相比,小鼠电离辐射后小肠Crp4表达水平升高,72h升高尤为显著(P<0.01);辐照小鼠血清LPS含量在9h后显著增加(P<0.05),72h后升高最为明显(P<0.01);Crp4对条件致病菌(大肠杆菌、粪肠球菌)的抗菌活性显著强于益生菌(乳酸杆菌、双歧杆菌),并能抑制LPS诱导单核巨噬细胞IL-1β的生成(P<0.01)。结论电离辐射可以诱导小鼠肠道隐窝素Crp4的表达上调,Crp4表达上调可发挥调节肠道菌群、减轻机体炎症反应的作用。

References

[1]  Neish A S. Microbes in gastrointestinal health and disease[J]. Gastroenterology, 2009, 136(1): 65-80. ? [2]吴小平, 贺杰. 肠道菌群失调与肠外疾病[J]. 医学新知杂志, 2014, 24(3): 188-189, 194. ? [3]席春晖, 冯志松. 肠道菌群在非酒精性脂肪性肝病发病机制中的研究进展[J]. 医学综述, 2014, 20(11): 1953-1955. ? [4]张英春, 韩雪, 单毓娟, 等. 益生菌抑制致病菌作用的机制研究进展[J]. 微生物学通报, 2012, 39(9): 1306-1313. ? [5]Rosengren K J, Daly N L, Fornander L M, et al. Structural and functional characterization of the conserved salt bridge in mammalian paneth cell-defensins: solution structures of mouse CRYPTDIN-4 and (E15D)-CRYPTDIN-4[J]. J Biol Chem, 2006, 281(38): 28068-28078. ? [6]Ayabe T, Satchell D P, Wilson C L, et al. Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria[J]. Nat Immunol, 2000, 1(2): 113-118. [7]Klotman M E, Chang T L. Defensins in innate antiviral immunity[J]. Nat Rev Immunol, 2006, 6(6): 447-456. ? [8]Crovella S, Antcheva N, Zelezetsky I, et al. Primate beta-defensins--structure, function and evolution[J]. Curr Protein Pept Sci, 2005, 6(1): 7-21. ? [9]Porter E M, Liu L, Oren A, et al. Localization of human intestinal defensin 5 in Paneth cell granules[J]. Infect Immun, 1997, 65(6): 2389-2395. [10]Satoh Y, Habara Y, Ono K, et al. Carbamylcholine- and catecholamine-induced intracellular calcium dynamics of epithelial cells in mouse ileal crypts[J]. Gastroenterology, 1995, 108(5): 1345-1356. ? [11]Gorbunov N V, Kiang J G. UP-regulation of autophagy in small intestine Paneth cells in response to total-body gamma-irradiation[J]. J Pathol, 2009, 219(2): 242-252. ? [12]刘霞, 梁振明, 杨景云, 等. ?60Co辐射后肠道菌群改变的实验研究[J]. 黑龙江医药科学, 2002, 25(3): 10. ? [13]Wilmes M, Cammue B P, Sahl H G, et al. Antibiotic activities of host defense peptides: more to it than lipid bilayer perturbation[J]. Nat Prod Rep, 2011, 28(8): 1350-1358. ? [14]Nuding S, Zabel L T, Enders C, et al. Antibacterial activity of human defensins on anaerobic intestinal bacterial species: a major role of HBD-3[J]. Microbes Infect, 2009, 11(3): 384-393. ? [15]Peschel A. How do bacteria resist human antimicrobial peptides?[J]. Trends Microbiol, 2002, 10(4): 179-186. ? [16]Rumio C, Besusso D, Palazzo M, et al. Degranulation of paneth cells via toll-like receptor 9[J]. Am J Pathol, 2004, 165(2): 373-381. ? [17]Shi J, Aono S, Lu W, et al. A novel role for defensins in intestinal homestasis: regulation of IL-1beta secretion[J]. J Immunol, 2007, 179(2): 1245-1253. ? [18]Ramasundara M, Leach S T, Lemberg D A, et al. Defensins and inflammation: the role of defensins in inflammatory bowel disease[J]. J Gastroenterol Hepatol, 2009, 24(2): 202-208. ? [19]Kelly P, Bajaj-Elliott M, Katubulushi M, et al. Reduced gene expression of intestinal alpha-defensins predicts diarrhea in a cohort of African adults[J]. J Infect Dis, 2006, 193(10): 1464-1470.

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