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大豆科学  2008 

农杆菌介导大豆子叶节系统的两个问题突破

DOI: 10.11861/j.issn.1000-9841.2008.01.0173

Keywords: 大豆,子叶节,丛生芽伸长,愈伤组织

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Abstract:

为解决大豆子叶节系统存在的再生和检测问题,对影响丛生芽伸长和植株检测的因素进行研究。以大豆品种黑农35的子叶节为外植体,利用L934正交试验确定影响再生系统丛生芽伸长的因素及水平,将转基因小植株的叶片培养愈伤组织以提取基因组DNA进行Southern检测。结果表明,丛生芽伸长的最佳组合为继代培养基与初始芽诱导培养基的6-BA浓度比为1/5,芽伸长培养基中添加50mgL-1Glu、0.5mgL-1GA3、0.5mgL-1ZT;Southern杂交信号说明利用叶片培养的愈伤组织能够提取足够量的基因组DNA。

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