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大豆科学  2009 

不同形态氮对大豆根瘤生长及固氮的影响

DOI: 10.11861/j.issn.1000-9841.2009.04.0674, PP. 674-677

Keywords: 根瘤,固氮酶活性,不同形态氮,大豆

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Abstract:

采用框栽的培养方法,通过施用不同形态氮肥,探讨不同形态氮对大豆根瘤生长、含氮量、氮积累量以及固氮酶活性的影响。结果表明:不同形态的氮源,对根瘤干重、数量有促进作用,同时增加了根瘤中的含氮量和氮积累量。根瘤干重及数量的变化均为铵态氮(N2)>蛋白氮(N4)>硝态氮(N1)>氨基酸态氮(N3)>酰胺态氮(N5)>生物固氮(N0),其中N0处理的根瘤干重、数量最少,而N2处理的根瘤干重和数量最多。根瘤含氮量的变化为N1>N4>N5>N2>N3>N0,其中N1处理下的根瘤中的根瘤含氮量最高,N1、N4和N5各处理与N0处理间差异显著(P<5%)。而氮积累的变化为N2>N4>N1>N5>N3>N0,各处理与N0处理相比,差异极显著(P<1%),与含氮量的变化趋势略有不同。添加不同形态的氮源对根瘤固氮酶活性有抑制作用,其变化趋势为N0>N5>N3>N2>N4>N1,N0处理的固氮酶活性最高。

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