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大豆科学  2008 

独角莲不同提取液对大豆蚜虫的生物活性及活性浓度的筛选

DOI: 10.11861/j.issn.1000-9841.2008.06.1010, PP. 1010-1014

Keywords: 独角莲,大豆蚜虫,均匀设计,生物活性

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Abstract:

基于均匀设计法研究了独角莲球根茎的不同提取液对大豆蚜虫的触杀和拒食作用。结果表明:乙醇浸提液对大豆蚜虫具有较高的触杀活性。水蒸汽蒸馏液对大豆蚜虫具有较强的拒食活性。乙醇浸提液对大豆蚜虫48h的致死中浓度(LC?50)为8.71mg.mL-1,水蒸汽蒸馏液对大豆蚜虫24、48h的拒食中浓度(AFC50)分别为8.66、7.93mg.mL-1。乙醚浸提液对大豆蚜虫的触杀和拒食作用不显著。60h的同时触杀和拒食活性浓度筛选的结果表明,乙醇浸提液24.1545mg.mL-1和水蒸汽蒸馏液23.014mg.mL-1联合使用对大豆蚜虫同时触杀和拒食活性最好,死亡率达100%。

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