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热休克转录因子1对2型糖尿病发病促进作用

DOI: 10.11847/zgggws2015-31-11-18, PP. 1427-1429

Keywords: 热休克转录因子1(HSF-1),2型糖尿病,热休克蛋白

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Abstract:

?目的探讨热休克转录因子1(HSF-1)在2型糖尿病发生发展中的作用及机制。方法SD大鼠随机分为2组:对照组和2型糖尿病模型组。分别采用Westernblot和RT-PCR方法检测2组大鼠HSF-1基因与蛋白表达水平,并在大鼠胰岛素细胞(INS-1)水平上检测HSF-1蛋白对于胰岛素分泌及相关信号通路活化影响。结果2型糖尿病模型大鼠HSF-1基因与蛋白表达分别上升209.3%和163.6%,腺苷酸活化蛋白激酶(AMPK)磷酸化水平明显提高。用SiRNA技术部分干扰INS-1细胞中HSF-1基因表达后,INS-1细胞在高糖环境培养中胰岛素分泌功能并未受到影响,但INS-1细胞中胰岛素受体的磷酸化水平提高。结论HSF-1可能参与了2型糖尿病的进程。

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