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北京地区医院污水中产NDM-1不动杆菌检测和抗生素敏感性分析

DOI: 10.11847/zgggws2015-31-08-30, PP. 1083-1086

Keywords: NDM-1,不动杆菌,耐药性

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Abstract:

?目的调查北京地区医院污水中含有NDM-1阳性的不动杆菌及耐药性情况。方法从北京多家医院采集污水标本,通过生化鉴定和16SrRNA测序进行病原分离鉴定;利用PCR技术对分离的病原进行blaNDM-1基因的鉴定;利用Sensititre药敏鉴定仪对NDM-1阳性菌进行抗生素敏感性分析。结果从医院污水中检出20株NDM-1阳性不动杆菌;所有菌对头孢类抗菌素、头孢西丁以及替卡西林和氨苄西林耐药率达到100%,有85%的菌对亚胺培南耐药。结论北京地区污水中产NDM-1阳性菌分离率较高,且主要为不动杆菌,其表现出多重耐药的特点,医院污水可能是NDM-1阳性菌存在的主要场所之一。

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