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草业学报  2011 

农杆菌介导的假俭草遗传转化体系的建立

, PP. 184-192

Keywords: 假俭草,农杆菌介导,ZjDREB1,遗传转化体系,建立

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Abstract:

以假俭草优良种质‘E126’为受体材料,以HPT基因为报告基因,通过农杆菌介导法转入ZjDREB1基因。研究了转化体系中筛选剂和抑菌素对胚性愈伤组织生长和绿苗分化的影响,以及菌液浓度、侵染时间和共培养时间对转化效率的影响。结果表明,愈伤筛选和再生苗筛选的最佳潮霉素浓度均为30mg/L,头孢霉素作为抑菌素的最佳浓度为400mg/L;菌液OD600值为0.3,侵染30min,共培养3d为最优转化条件。经PCR检测,初步获得10株转基因植株。

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