全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2011 

马铃薯WRKY6基因的克隆、序列分析与原核表达研究

, PP. 177-183

Keywords: 马铃薯,WRKY,克隆,序列分析,原核表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

在高等植物中WRKY转录因子家族成员众多,它广泛参与植物对生物胁迫、非生物胁迫、生长发育和代谢过程的调控。本研究采用同源序列克隆的方法获得马铃薯WRKY6基因,序列分析表明该蛋白属于WRKY家族第3组成员,锌指结构为C-X7-C-X23-H-X-C。构建系统发育树结果表明它与拟南芥WRKY70亲缘关系较近,相似性达58%。然后将其完整编码区构建到大肠杆菌表达载体。在培养温度18℃条件下添加0.2mmol/LIPTG(异丙基硫代半乳糖苷),诱导培养8h可获得高表达融合蛋白。进一步实验获得特异性和纯度非常高的纯化蛋白。本研究为进一步确定该蛋白的体外活性及生物学功能奠定了坚实基础。

References

[1]  Wang J, Zhang S, Stacey G. Activation of a mitogen-activated protein kinase pathway in Arabidopsis by chitin. Molecular Plant Pathology, 2004, 5: 125-135.
[2]  Robatzek, Somssich I E. Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes Development, 2002, 16: 1139-1149.
[3]  Jonson C S, Kolevski B, Smyth D R. TRANSPARENT TESTA GLA-BRA 2, a trichome and seed coat development gene of Arabidopsis, encode a WRKY transcription factor. Plant Cell, 2002, 14: 1359-1375.
[4]  Luo M, Dennis E S, Berger F, et al. A MINI SEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucine-rich repeat (LRR) kinase gene, are regulators of seed size in Arabidopsis. Proceedings of the National Academy of Sciences USA, 2005, 102: 17531-17536.
[5]  Mare C, Mazzucotelli E, Crosatti C, et al. Hv-WRKY38: A new transcription factor involved in cold and drought response in barley. Plant Molecular Biology, 2004, 55: 399-416.
[6]  Devaiah B N, Karthikeyan A S, Raghothama K G. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiology, 2007, 143: 1789-1801.
[7]  ChenY F, Li L Q, Xu Q, et al. The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis. The Plant Cell, 2009, 21: 3554-3566.
[8]  程天庆. 马铃薯栽培技术(第2版). 北京: 金盾出版社, 2004.
[9]  Eulgem T, Rushton P J, Robatzek S, et al. The WRKY superfamily of plant transcriptional factor. Trends Plant Science, 2000, 5(5): 199-206.
[10]  Li J, Brader G, Palva E T. The WRKY70 transcription factor: A node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell, 2004, 16(2): 319-331.
[11]  郑轶琦, 刘建秀. 草坪草分子遗传图谱的构建与应用研究进展. 草业学报, 2009, 18(1): 155-162.
[12]  尚以顺, 杨春燕, 钟理. 贵州野生匍匐剪股颖种质资源遗传多样性的ISSR分析. 草业学报, 2009, 18(3): 67-73. 浏览
[13]  沈宝云, 刘玉汇, 张俊莲, 等. 马铃薯块茎GBSSⅠ基因的cDNA克隆及其序列特征分析. 草业学报, 2010, 19(5): 1-8. 浏览
[14]  Yamasaki K, Kigawa T, Inoue M, et al. Soltuion structeru of an Arabidopsis WRKY DNA bidning doamin. Plant Cell, 2005, (37): 944-956.
[15]  Duan M R, Nan J, Liang Y H, et al. DNA binding mechanism revealed by high resolution crystal structure of Arabidopsis thaliana WRKY1 protein. Nucleic Acids Research, 2007, 35: 1145-1154.
[16]  Yang B, Jiang Y, Rahman M H, et al. Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments. BMC Plant Biology, 2009, 9: 68-87.
[17]  张兰, 王晓萍, 毕影东, 等. 大豆转录因子GmWRKY57B的基因克隆及功能分析. 科学通报, 2008, 53(21): 2604-2611.
[18]  Dellagi A, Helibronn J, Avrova A O, et al. A potato gene encoding a WRKY-like transcription factor is induced in interactions with Erwinia carotovora subsp. atroseptica and Phytophthora infestans and is coregulate with classI endochitinase expression. Molecular Plant Microbe Interactions, 2000, 13(10): 1092-1101.
[19]  Ishiguro S, Nakamura K. Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the upstream regions of genes coding for sporamin and beta-amylase from sweet potato. Molecular and General Genetics, 1994, 244(6): 563-571.
[20]  Robatzek S, Somssich I E. A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence-and defence-related processes. Plant Journal, 2001, 28: 123-133.
[21]  Lagacé M, Matton D P. Characterization of a WRKY transcription factor expressed in late torpedo stage embryos of Solanum chacoense. Planta, 2004, 219: 185-189.
[22]  Xu Y H, Wang J W, Wang S, et al. Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-δ-cadinene synthase-A. Plant Physiology, 2004, 135: 507-515.
[23]  Zhang Z L, Xie Z, Zou X L, et al. A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiology, 2004, 134: 1500-1513.
[24]  Chen C, Chen Z. Isolation and characterization of two pathogen and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco. Plant Molecular Biology, 2000, 42: 387-396.
[25]  Sun C X, Palmqvist S, Olsson H, et al. A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of theiso1 promoter. Plant Cell, 2003, 15: 2076-2092.
[26]  Hwang E W, Kim K A, Park S C, et al. Expression profiles of hot pepper (Capsicum annum) genes under cold stress conditions. Journal of Biosciences, 2005, 30(5): 657-667.
[27]  Wei W, Zhang Y X, Han L, et al. A novel WRKY transcriptional factor from thlaspi caerulescens negatively regulates the osmotic stress tolerance of transgenic Tobacco. Plant Cell Reports, 2008, 27(4): 795-803.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133