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草业学报  2012 

不同精粗饲料对泌乳中期山羊氨基酸的代谢流向及乳蛋白含量的影响

, PP. 258-265

Keywords: 不同日粮,泌乳中期山羊,血浆,乳,乳蛋白,游离氨基酸

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Abstract:

通过检测泌乳中期山羊血浆及乳中游离氨基酸含量的变化,探讨不同精粗比饲料对机体中氨基酸(aminoacids,AA)的代谢流向及乳蛋白的影响。选用泌乳中期山羊7只,随机分为2组,分别饲喂精粗比4∶6(低精料组)和6∶4(高精料组)日粮,颈静脉血管瘘采血,手工挤奶取奶样。Bradford法测定乳中总蛋白质浓度,RP-HPLC检测山羊血浆和乳中游离氨基酸的含量。结果表明,2组山羊血浆中均分离出14种游离氨基酸,乳中分离出8种游离氨基酸,不同精粗比饲料对血液或乳中游离氨基酸谱无明显影响。不同精粗比日粮情况下,除丙氨酸外,血液中游离氨基酸的含量均高于乳中同种氨基酸的含量;且血液中生酮氨基酸(Leu,Lys)、兼性氨基酸(Phe,Ile)和支链氨基酸(Leu,Ile和Val)含量显著或极显著高于乳中。高精料组除Gly和Ala外,其余氨基酸含量均低于低精料组;乳中总蛋白浓度略高于低精料组,但无统计学差异。表明血液中游离氨基酸为乳蛋白的合成提供了原料和能量保证。饲喂高精料日粮,血液中游离氨基酸含量较低,不利于乳蛋白含量和产量的提高。

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