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草业学报  2015 

氮对千日红试管苗生长和开花诱导的影响

DOI: 10.11686/cyxb20150108, PP. 56-63

Keywords: 千日红,试管苗,氮(N),生长,试管开花

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Abstract:

本文以千日红组培苗为试验材料,研究培养基中氮形态及含量对千日红试管苗生长和开花诱导的影响。结果表明,1)相对于铵态氮(NH4+),硝态氮(No3-)作为唯一氮源更有利于千日红试管苗生长和开花诱导,但千日红在NH4+和NO3-同时存在的培养基中表现最佳。2)在20mmol/LNH4+(NO3-)和5mg/LPP333存在的条件下,试管苗生长基本随着培养基中NO3-(NH4+)含量的增加而增加,并在含40mmol/LNO3-+20mmol/LNH4+(即MS培养基中氮含量)的培养基中株高达到最大值5.91cm;而叶片数和开花率则随着培养基中NH4+和NO3-含量的增加呈现先增加后下降的趋势,并在20mmol/LNO3-+5mmol/LNH4+培养基中达到最大值,分别为10.7片/株和38.89%。3)氮含量及形态配比结果表明,千日红试管苗开花率在培养基中氮总量为5mmol/L、NO3-/NH4+为4/1时达到最大值39.95%,而株高和叶片数在氮总量为35mmol/L,NO3-/NH4+为4/1时达到最大值8.52cm和13.38片/株。千日红试管苗开花率与培养基中NO3-/NH4+显著正相关,而与氮总量及株高之间显著负相关。此外,培养基中氮含量及形态配比还显著影响无菌苗根系生长。

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