HPLC同时测定北五味子中5种木脂素的含量

目的:建立同时测定北五味子中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素含量的方法。方法:以SymmetryC18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水,梯度洗脱,0~36min(60∶40~66∶34);36~65min(66∶34~80∶20);65~70min(80∶20),70~75min(80∶20~100∶0),流速0.5mL·min-1,检测波长254nm。结果:五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素和五味子乙素分别在0.021~4.160,0.020~4.000,0.021~4.240,0.020~3.960,0.021~4.200μg呈良好的线性关系(r=0.9999);平均加样回收率分别为104.8%,104.2%,102.7%,104.6%,104.5%。RSD值均小于3%。结论:本方法稳定易行、重复性好,可用于北五味子药材的质量评价。