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左金丸抗肿瘤作用及对S180荷瘤小鼠血清肿瘤标志物的影响

Keywords: S180,黄连,吴茱萸,左金丸,肿瘤标志物

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Abstract:

目的:研究左金丸的体内抗肿瘤作用,并探讨其对S180荷瘤小鼠血清中肿瘤标志物酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、肌酸激酶(CK)、醛缩酶(ALD)和乳酸脱氢酶(LDH)活力的影响,并与其配伍方药黄连和吴茱萸进行比较。方法:建立S180实体瘤模型,昆明种小鼠右腋下接种0.2mLS180细胞悬液(5×106个/mL细胞)后,随机分成5组,24h后分别给予左金丸(850.8mg·kg-1)、黄连(729.2mg·kg-1)和吴茱萸(121.6mg·kg-1)提取物10d,观察小鼠体重、脾指数,计算抑瘤率和生命延长率(ILS),并检测不同组给药小鼠血清中ACP,AKP,CK,ALD和LDH的活力。结果:左金丸对移植性S180肿瘤的抑瘤率为50.54%,对小鼠的ILS为64.91%。同时左金丸可显著提高小鼠血清ACP(126.72±11.16)U·100mL-1和AKP(67.27±13.49)U·100mL-1的活力,且显著降低小鼠血清CK(20.65±4.28)U·mL-1,ALD(319.13±53.87)U·L-1和LDH(1029.04±468.56)U·L-1的活力,与黄连和吴茱萸单独给药有着显著性差异(P<0.01)。结论:左金丸方中黄连和吴茱萸能产生明显的配伍协同抗肿瘤作用,对血清5种肿瘤标志物的影响可能是其抗肿瘤作用的潜在机制。

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