全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

代谢组学在中药现代研究的应用与展望

Keywords: 代谢组学,中药,机制

Full-Text   Cite this paper   Add to My Lib

Abstract:

代谢组学是研究机体代谢网络和代谢产物整体变化及内在变化规律的重要方法,与中药学的研究思路有一定的相似性。在中药现代化的研究过程中,充分借鉴代谢组学的研究方法,将加快传统中药学与现代生命科学的结合,推动中药现代化的发展。该文主要从代谢组学的概念、代谢组学在中药毒性作用机制、中药方剂配伍机制、中药方剂药效作用机制和中药炮制机制等研究中的应用予以阐述。总结了代谢组学在中药现代研究中存在的问题,并展望了发展前景。

References

[1]  Gong P, Cui N, Wu L, et al. Chemicalome and metabolome matching approach to elucidating biological metabolic networks of complex mixtures[J]. Anal Chem, 2012, 84(6):2995.
[2]  Li X, Luo X X, Lu X, et al. Metabolomics study of diabetic retinopathy using gas chromatography-mass spectrometry: a comparison of stages and subtypes diagnosed by Western and Chinese medicine[J]. Mol Biosyst, 2011, 7(7):2228.
[3]  王广基,查伟斌,郝海平,等.代谢组学技术在中医药关键科学问题研究中的应用前景分析[J].中国天然药物,2008,6(2):89.
[4]  Chen M. Metabonomic study of aristolochic acid-induced nephrotoxicity in rats[J]. J Proteome Res, 2006, 5(4):995.
[5]  杨琪伟,杨莉,熊爱珍,等.赤芍和白芍抗炎作用的UPLC-MS代谢组学初步研究[J].中国中药杂志,2011,36(6):694.
[6]  薛水玉,王雪洁,孙海峰,等.基于气质联用的款冬花蕾动态发育代谢组学特征分析[J].中国中药杂志,2012,37(19):2863.
[7]  郭晓擎,田俊生,史碧云,等.南柴胡和北柴胡组成的逍遥散抗抑郁作用的1H-NMR代谢组学研究[J].中草药,2012,43(11):2209.
[8]  Xiang Z, Wang X Q, Cai X J, et al. Metabolomics study on quality control and discrimination of three Curcuma species based on gas chromatograph-mass spectrometry[J]. Phytochem Anal, 2011, 22(5):411.
[9]  Sun H, Wang M, Zhang A, et al. UPLC-Q-TOF-HDMS analysis of constituents in the root of two kinds of Aconitum using a metabolomics approach[J]. Phytochem Anal, 2013, 24(3):263.
[10]  Li Y Y, Wang X X, Zhao L, et al. High-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry analysis of Radix Saposhnikoviae for metabolomic research[J]. J Chromatogra Sci, 2012,10:1.
[11]  龚梦鹃,叶文华,谢媛媛,等.巴戟天补肾阳作用的血清代谢组学研究[J].中国中药杂志,2012,37(11):1682.
[12]  张上上,刘心昱,郑姝宁,等.骨碎补醇提物对骨质疏松预防作用的大鼠尿液UPLC-MS/MS代谢组学研究[J].中国中药杂志,2012,37(5):658.
[13]  王东琴,李晓伟,张福生,等.基于GC-MS代谢组学技术的狭叶柴胡解热作用研究[J].中草药,2013,44(5):574.
[14]  李春雨,王平,王张,等.基于代谢组学技术的大黄治疗慢性肾功能衰竭的作用机制研究[J].中草药,2012,43(2):312.
[15]  谷金宁,牛俊,皮子凤,等.尿液代谢组学方法研究人参总皂苷治疗糖尿病心肌病大鼠作用机制[J].分析化学,2013,41(3):371.
[16]  Zhao Y Y, Feng Y L, Bai X, et al. Ultra performance liquid chromatography-based metabonomic study of therapeutic effect of the surface layer of Poria cocos on adenine-induced chronic kidney disease provides new insight into anti-fibrosis mechanism[J]. PLoS ONE,2013,doi: 10.1371/journal.pone.0059617.
[17]  Qiao X, Ye M, Xiang C, et al. Metabolic regulatory effects of licorice: a bile acid metabonomic study by liquid chromatography coupled with tandem mass spectrometry[J]. Steroids, 2012,77:745.
[18]  Lu X, Xiong Z, Li J, et al. Metabonomic study on \'Kidney-Yang Deficiency syndrome\' and intervention effects of Rhizoma Drynariae extracts in rats using ultra performance liquid chromatography coupled with mass spectrometry[J]. Talanta, 2011, 83(3):700.
[19]  Qi Y, Qu L, Wu Y, et al. A plasma metabonomic investigation into the intervention of volatile oil of Magnolia biondii pamp on rat model of acute inflammation[J]. J Ethnopharmacol, 2011, 137:487.
[20]  Yan S, Wu B, Lin Z, et al. Metabonomic characterization of aging and investigation on the anti-aging effects of total flavones of Epimedium[J]. Mol Biosyst, 2009, 5(10):1204.
[21]  郭娜,范斌,彭娟,等.基于超高效液相-飞行时间质谱技术的中药女贞子代谢组学研究[J].中国实验方剂学杂志,2010,16(10):131.
[22]  王雪洁,李震宇,薛水玉,等.基于植物代谢组学技术的远志不同炮制品质量控制研究[J].中草药,2012,43(9):1727.
[23]  Geng L, Ma C, Zhang L, et al. Metabonomic study of Genkwa Flos-induced hepatotoxicity and effect of herb-processing procedure on toxicity[J]. Phytother Res, 2013, 27(4):521.
[24]  Li Y, Wang Y, Su L, et al. Exploring potential chemical markers by metabolomics method for studying the processing mechanism of traditional Chinese medicine using RPLC-Q-TOF/MS: a case study of Radix Aconiti[J]. Chem Cent J, 2013, 7(1):36.
[25]  Zhang H M, Li S L, Zhang H, et al. Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach[J]. J Pharm Biomed Anal, 2012, 62:258.
[26]  梁琦,谢鸣,倪诚,等.广防己配伍黄芪肾毒性的代谢组学研究[J].浙江中医药大学学报,2010,34(1):42.
[27]  柳长凤,陈平平,刘树民,等.基于代谢组学技术的黄连解毒汤配伍规律研究[J].中国实验方剂学杂志,2012,18(13):147.
[28]  武斌, 曹敏, 于海龙, 等. 基于代谢组学的苍耳子配伍黄芪后减毒作用研究[J].中药药理与临床, 2012, 28(2):98.
[29]  李欢,夏鑫华,刘梅,等.麝香配伍冰片干预局灶性脑缺血-再灌注损伤的代谢组学研究[J].中药材,2012,35(8):1274.
[30]  王静,袁子民,李云兴,等.基于GC-MS代谢组学法研究黄连、生地黄治疗Ⅱ型糖尿病的配伍机制[J].中国中药杂志,2014,39(3):526.
[31]  施旭光,吴美音,黄曼婷,等.基于代谢组学的补中益气汤"益气升阳"配伍机制研究[J].中国实验方剂学杂志,2014,20(1):103.
[32]  Wang H, Bai J, Chen G, et al. A metabolic profiling analysis of the acute hepatotoxicity and nephrotoxicity of Zhusha Anshen Wan compared with cinnabar in rats using 1H NMR spectroscopy[J]. J Ethnopharmacol, 2013, 146(2):572.
[33]  陈曦,杨永霞,王淑美,等.基于NMR代谢组学研究脑脉通复方提取物对大鼠脑缺血再灌注模型的影响[J].中草药,2012,43(1):97.
[34]  王彬,沈岚,从文娟,等.基于代谢组学的六味地黄丸干预大鼠甲亢模型的作用研究[J].中国药理学通报,2013,29(5):632.
[35]  吴宏伟,高健,李韶菁,等.基于液相色谱-串联质谱的氨基酸代谢组学方法研究黄芪注射液治疗脑缺血[J].分析化学,2013,41(3):344.
[36]  涂佳玉,阿基业,王广基,等.通塞脉微丸干预缺血性中风模型大鼠的血浆代谢组学研究[J].中国中药杂志,2012,37(7):1028.
[37]  Su Z H, Li S Q, Zou G A, et al. Urinary metabonomics study of anti-depressive effect of Chaihu-Shu-Gan-San on an experimental model of depression induced by chronic variable stress in rats[J]. J Pharm Biomed Anal, 2011, 55:533.
[38]  Yue R, Zhao L, Hu Y, et al. Metabolomic study of collagen-induced arthritis in rats and the interventional effects of Huang-Lian-Jie-Du-Tang, a traditional Chinese medicine[J]. Evid Based Complement Alternat Med, 2013, doi: 10.1155/2013/439690.
[39]  Jiang H, Nie L, Li Y, et al. Application of ultra-performance liquid chromatography coupled with mass spectrometry to metabonomic study on spontaneously hypertensive rats and intervention effects of Ping Gan prescription[J]. J Sep Sci, 2012, 35(4):483.
[40]  Xiang L, Jiang P, Zhan C, et al. The serum metabolomic study of intervention effects of the traditional Chinese medicine Shexiang Baoxin Pill and a multi-component medicine polypill in the treatment of myocardial infarction in rats[J]. Mol Biosyst, 2012, 8(9):2434.
[41]  Liang X, Chen X, Liang Q, et al. Metabonomic study of Chinese medicine shuanglong formula as an effective treatment for myocardial infarction in rats[J]. J Proteome Res, 2011, 10(2):790.
[42]  Tan G, Liao W, Dong X, et al. Metabonomic profiles delineate the effect of traditional Chinese medicine Sini decoction on myocardial infarction in rats[J]. PLoS ONE, 2012, doi: 10.1371/journal.pone.0034157.
[43]  Dai W, Wei C, Kong H, et al. Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry[J]. J Pharm Biomed Anal, 2011, 56(1):86.
[44]  Wang X, Wang Q, Zhang A, et al. Metabolomics study of intervention effects of Wen-Xin-Formula using ultra high-performance liquid chromatography/mass spectrometry coupled with pattern recognition approach[J]. J Pharm Biomed Anal, 2013, 74:22.
[45]  Gou X, Tao Q, Feng Q, et al. Urinary metabonomics characterization of liver fibrosis induced by CCl4 in rats and intervention effects of Xia Yu Xue decoction[J]. J Pharm Biomed Anal, 2013, 74:62.
[46]  Gao X X, Cui J, Zheng X Y, et al. An investigation of the anti-depressant action of Xiaoyaosan in rats using ultra performance liquid chromatography-mass spectrometry combined with metabonomics[J]. Phytother Res, 2013, 27(7):1074.
[47]  Song X, Wang J, Wang P, et al. 1H NMR-based metabolomics approach to evaluate the effect of Xue-Fu-Zhu-Yu decoction on hyperlipidemia rats induced by high-fat diet[J]. J Pharm Biomed Anal, 2013(78/79):202.
[48]  Zhao L, Gao H, Zhao Y, et al. Metabonomic analysis of the therapeutic effect of Zhibai Dihuang pill in treatment of streptozotocin-induced diabetic nephropathy[J]. J Ethnopharmacol, 2012, 142:647.
[49]  王丽, 宋敏, 杭太俊, 等. NMR代谢组学法研究大蒜辣素对大鼠的作用机制[J].药学学报, 2009, 44 (9): 1019.
[50]  Gu Y, Zhang Y, Shi X, et al. Effect of traditional Chinese medicine berberine on type 2 diabetes based on comprehensive metabonomics[J]. Talanta, 2010, 81(3):766.
[51]  Zhang A, Sun H, Dou S, et al. Metabolomics study on the hepatoprotective effect of scoparone using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry[J]. Analyst, 2013,138(1):353.
[52]  梁晓萍,张政,胡坪,等.蟾酥急性毒性的代谢组学研究[J].高等学校化学学报,2011,32(1):38.
[53]  王亮,江涛,冯毅凡,等.代谢组学评价蛇床子提取物对大鼠毒理作用的初步研究[J].亚太传统医药,2010,6(10):20.
[54]  Ma C, Bi K S, Zhang M, et al. Toxicology effects of morning glory seed in rat: a metabonomic method for profiling of urine metabolic changes[J]. J Ethnopharmacol, 2010, 130(1):134.
[55]  杨永霞,唐冰雯,丁佳佳,等. 甘遂毒性的血浆代谢组学研究[J]. 第三军医大学学报, 2014, 36(1):38.
[56]  Yan G, Zhao Y, Deng P, et al. Investigation of toxicological effects of Shuanghuanglian injection in Beagle dogs by metabonomic and traditional approaches[J]. Exp Biol Med (Maywood),2010,235(11):1356.
[57]  Liu Y, Huang R, Liu L, et al. Metabonomics study of urine from Sprague-Dawley rats exposed to Huang-yao-zi using 1H-NMR spectroscopy[J]. J Pharm Biomed Anal,2010, 52(1):136.
[58]  Ma C, Bi K, Su D, et al. Serum and kidney metabolic changes of rat nephrotoxicity induced by morning glory seed[J]. Food Chem Toxicol, 2010, 48(10):2988.
[59]  Xu W, Wang H, Chen G, et al. 1HNMR-based metabonomics study on the toxicity alleviation effect of other traditional Chinese medicines in Niuhuang Jiedu tablet to realgar (As2S2)[J]. J Ethnopharmacol, 2013,148(1):88.
[60]  Nicholson J K, Lindon J C, Holmes E. ″Metabonomics″: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenbiotica, 1999, 29(11):1181.
[61]  Shyur L F, Yang N S. Metabolomics for phytomedicine research and drug development[J]. Curr Opin Chem Biol, 2008, 12(1):66.
[62]  Clayton T A, Lindon J C, Cloarec O, et al. Pharmaco-metabonomic phenotyping and personalized drug treatment[J]. Nature, 2006, 440(7087):1073.
[63]  Lao Y M, Jiang J G, Yan L. Application of metabonomic analytical techniques in the modernization and toxicology research of traditional Chinese medicine[J]. Br J Pharmacol, 2009, 157(7):1128.
[64]  Zhao L P, Nicholson J K, Lu A P, et al. Targeting the human genome-microbiome axis for drug discovery:inspirations from global systems biology and traditional Chinese medicine[J]. J Proteome Res, 2012, 11(7):3509.
[65]  Ebbels T M. Prediction and classification of drug toxicity using probabilistic modeling of temporal metabolic data:the consortium on metabonomic toxicology screening approach[J]. J Proteome Res, 2007, 6(11):4407.
[66]  Wang X J , Wang H Y , Zhang A H , et al. Metabolomics study on the toxicity of Aconite root and its processed products using ultra performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry coupled with pattern recognition approach and ingenuity pathways analysis[J]. J Proteome Res, 2012, 11(2):1284.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133