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深海放线菌Micrococcussp.R21的次生代谢产物研究

Keywords: 深海,放线菌,微球菌,次生代谢产物,抗肿瘤

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Abstract:

研究一株来源于西太平洋深海沉积环境(-6310m)的放线菌菌株Micrococcussp.R21的抗肿瘤活性次生代谢产物。结合运用用硅胶,凝胶柱色谱等方法从其菌液的乙酸乙酯萃取物中分离鉴定了8个化合物,通过核磁数据结合其他波谱数据鉴定其结构分别为:环(4-羟基-L-脯-L-亮)二肽(1),及环(L-脯-L-甘)二肽(2),环(L-脯-L-丙)二肽(3),环(D-脯-L-亮)二肽(4),N-β-乙酰色胺酸(5),邻羟基苯甲酸(6),2-苯基乙酸(7)。其中化合物1对小鼠单核巨噬细胞白血病细胞RAW264.7显示出较好的生长抑制活性(IC50=9.1μmol·L-1),提示1可能是该菌抗肿瘤的活性成分。

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