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绞股蓝属植物亲缘关系初步分析

Keywords: 绞股蓝,ITS,序列分析,系统发育树

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Abstract:

采用DNA条形码ITS,matK,rbcL,psbA-trnH4个片段,对绞股蓝属Gynostemma9个种及变种共38份样品的序列进行分析,以雪胆属罗锅底Hemsleyamacrosperma作为外类群,运用PAUP4.0b10软件进行系统发育分析,基于ITS序列采用邻接法(NJ)和最大简约法(MP)构建系统发育树,探讨绞股蓝属下的亲缘关系.研究表明:①在NJ树和严格一致树中,广布种绞股蓝G.pentaphyllumvar.pentaphyllum的8个个体没有形成单系分支;②怀疑长梗绞股蓝G.longipes和光叶绞股蓝G.laxum是否应为独立的种;③支持绞股蓝属植物亚属的分类单位.

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