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植物研究  2015 

水稻OsLOL2基因的克隆及过表达拟南芥的抗盐性分析

DOI: 10.7525/j.issn.1673-5102.2015.02.014, PP. 259-269

Keywords: LSD-Like基因家族,转录因子,OsLOL2基因,表达模式分析,非生物逆境胁迫

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Abstract:

?从水稻龙粳11(OryzasativaL.longjing11)中克隆到了一个已知的LSD1-Like型锌指蛋白转录因子OsLOL2基因(LOC_Os12g41700)。生物信息学分析表明,OsLOL2基因ORF长度为519bp,编码172个氨基酸残基,有2个LSD1-Like锌指结构域。本研究以基因枪转化洋葱表皮细胞定位表明在核中。实时荧光定量PCR检测到OsLOL2基因在龙粳11叶和根中的表达受80mmol·L-1NaCl、30mmol·L-1NaHCO3、10mmol·L-1H2O2胁迫诱导而转录表达显著增加。过表达OsLOL2基因酵母表现出抗盐碱性生长;在胁迫条件下,萌发率分析表明,转OsLOL2拟南芥在NaCl和H2O2下,过表达株系萌发率高于野生型;萌发后期抗性检测表明:在含100mmol·L-1NaCl、3mmol·L-1NaHCO3和2mmol·L-1H2O2的1/2MS平板上,过表达株系长势优于野生型,揭示了OsLOL2基因能够提高植物的抗盐碱性,与盐碱逆境引起的氧化胁迫的ROS途径相关。为OsLOL2基因参与逆境信号途径的研究提供了理论依据。

References

[1]  1.Schneiderm,Tognollim,Bairocha.The Swiss Protprotein knowledge base and ExPASy:providing the plant community with high quality proteomic data and tools[J].Plant Physiol Biochem,2004(42):1013-1021.
[2]  2.Miller J,McLachlan A D,Klug A.Repetitive zincbinding domains in the protein transcription factor ⅢA from Xenopus oocytes[J].The EMBO journal,1985(4):1609.
[3]  3.Mittler Ron,Vanderauwera Sandy,Gollery Martin,et al.Reactive oxygen gene network of plants[J].Trends in plant science,2004(9):490-498.
[4]  4.Huang Ji,Sun Shujing,Xu Dongqing,et al.Increased tolerance of rice to cold,drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245[J].Biochemical and biophysical research communications,2009(389):556-561.
[5]  5.Huang Ji,Yang Xia,Wang Meimei,et al.A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance[J].Biochimica et Biophysica Acta(BBA)Gene Structure and Expression,2007(1769):220-227.
[6]  6.Islam M S,Hur J H,Wang M H.The influence of abiotic stresses on expression of zinc finger protein gene in rice[J].Russian Journal of Plant Physiology,2009(56):695-701.
[7]  7.Jain Mukesh,Tyagi Akhilesh K,Khurana Jitendra P.Constitutive expression of a meiotic recombination protein gene homolog,OsTOP6A1,from rice confers abiotic stress tolerance in transgenic Arabidopsis plants[J].Plant cell reports,2008(27):767-778.
[8]  8.黄越敏,胡红红,武从庆.一个逆境诱导表达的水稻锌指蛋白基因的分离和鉴定[J].华中农业大学学报,2007(25):581-585.
[9]  9.Huang Ji,Wang Meimei,Jiang Yan,et al.Expression analysis of rice A20/AN1-type zinc finger genes and characterization of ZFP177 that contributes to temperature stress tolerance[J].Gene,2008(420):135-144.
[10]  10.Ciftci-Yilmaz Sultan,Morsy Mustafa R,Song Luhua,et al.The EAR-motif of the Cys2/His2-type zinc finger protein Zat7 plays a key role in the defense response of Arabidopsis to salinity stress[J].Journal of Biological Chemistry,2007(282):9260-9268.
[11]  11.Barth Olaf,Vogt Sebastian,Uhlemann Ria,et al.Stress induced and nuclear localized HIPP26 from Arabidopsis thaliana interacts via its heavy metal associated domain with the drought stress related zinc finger transcription factor ATHB29[J].Plant molecular biology,2009(69):213-226.
[12]  12.Kang Miyoung,Fokar Mohamed,Abdelmageed Haggag,et al. Arabidopsis SAP5 functions as a positive regulator of stress responses and exhibits E3 ubiquitin ligase activity[J].Plant molecular biology,2011(75):451-466.
[13]  13.Milla Miguel A Rodriguez,Townsend Jared,Chang Feng,et al.The Arabidopsis AtDi19 gene family encodes a novel type of Cys2/His2 zinc-finger protein implicated in ABA-independent dehydration,high-salinity stress and light signaling pathways[J].Plant molecular biology,2006(61):13-30.
[14]  14.Liu Qinglin,Xu Kedong,Ma Nan,et al.Isolation and functional characterization of DgZFP:a gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum[J].Molecular biology reports,2010(37):1137-1142.
[15]  15.Chao Yuehui,Kang Junmei,Sun Yan,et al.Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.[J].Molecular biology reports,2009(36):2315-2321.
[16]  16.Wang Huahua,Huang Junjun,Bi Yurong.Nitrate reductase-dependent nitric oxide production is involved in aluminum tolerance in red kidney bean roots[J].Plant science,2010(179):281-288.
[17]  17.Tian Z D,Zhang Y,Liu J,et al.Novel potato C2H2-type zinc finger protein gene,StZFP1,which responds to biotic and abiotic stress,plays a role in salt tolerance[J].Plant Biology,2010(12):689-697.
[18]  18.Li Cuiling,Lü Jian,Zhao Xin,et al.TaCHP:a wheat zinc finger protein gene down-regulated by abscisic acid and salinity stress plays a positive role in stress tolerance[J].Plant Physiology,2010(154):211-221.
[19]  19.Ham ByungKook,Park Jeong Mee,Lee SukBae,et al.Tobacco Tsip1,a DnaJ-type Zn finger protein,is recruited to and potentiates Tsi1-mediated transcriptional activation[J].The Plant Cell Online,2006(18):2005-2020.
[20]  20.Xu Chunxiao,He Chaozu.The rice OsLOL2 gene encodes a zinc finger protein involved in rice growth and disease resistance[J].Molecular Genetics and Genomics,2007(278):85-94.
[21]  21.Kei?a Anete,Kānberga Krista,Gill Upinder,et al.Cloning and characterization of barley homologues of the Arabidopsis LSD1 gene:putative regulators of hypersensitive response[J].Acta Univ Latviensis,2008(745):87-101.
[22]  22.白鹏飞,杨倩,康振生,等.小麦锌指蛋白基因 TaLOL2的克隆及特征分析[J].西北植物学报,2012(32):2151-2156.
[23]  23.Wang Lijuan,Pei Zhongyou,Tian Yingchuan,et al.OsLSD1,a rice zinc finger protein,regulates programmed cell death and callus differentiation[J].Molecular plantmicrobe interactions,2005(18):375-384.
[24]  24.Bhatti Khizar Hayat,Shah Amin,Iqbal Mazhar,et al.Transgenic tobacco with rice zinc-finger gene OsLOL2 exhibits an enhanced resistance against bacterial-wilt[J].Australasian Plant Pathology,2011(40):133-140.
[25]  25.Bhatti Khizar Hayat,Xu Chunxiao,Wu Jiahe,et al.Overexpression of rice OsLOL2 gene confers disease resistance in tobacco to Pseudomonas syringae pv. tabaci[J].Progress in Natural Science,2008(18):807-812.
[26]  26.Alinsug Malona V,Chen Fangfang,Luo Ming,et al.Subcellular localization of class Ⅱ HDAs in Arabidopsis thaliana:nucleocytoplasmic shuttling of HDA15 is driven by light[J].Plo Sone,2012(7):30846.
[27]  27.Sambrook Joseph,Fritsch Edward F,Maniatis Thomas.Molecular cloning:Cold spring harbor laboratory press New York,1989.
[28]  28.Hsieh Tsai-Hung,Li Chiawen,Su Ruey-Chih,et al.A tomato bZIP transcription factor,SlAREB,is involved in water deficit and salt stress response[J].Planta,2010(231):1459-1473.
[29]  29.Mukhopadhyay Arnab,Vij Shubha,Tyagi Akhilesh K.Overexpression of a zinc-finger protein gene from rice confers tolerance to cold,dehydration,and salt stress in transgenic tobacco[J].Proceedings of the National Academy of Sciences of the United States of America,2004(101):6309-6314.
[30]  30.Hasegawa P M,Bressan R A,Zhu J K,et al.Plant cellular and molecular responses to high salinity[J].Ann Rev Plant Physiol Plant Mol Biol,2000(51):463-499.
[31]  31.Liu Xiaoli,Liu Shenkui,Takano Tetsuo.碱茅Put-HVA1基因的克隆及在酵母和拟南芥中的表达[J].分子植物育种,2010(5):846-852.
[32]  32.Giniger Edward,Varnum Susan M,Ptashne Mark.Specific DNA binding of GAL4,a positive regulatory protein of yeast[J].Cell,1985(40):767-774.
[33]  33.Cunningham Kyle W,Fink Gerald R.Calcineurin inhibits VCX1-dependent H +/Ca 2+ exchange and induces Ca 2+ ATPases in Saccharomyces cerevisiae[J].Molecular and Cellular Biology,1996(16):2226-2237.

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