全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
植物研究  2015 

番茄转录因子SlYABBY5的克隆与表达分析

DOI: 10.7525/j.issn.1673-5102.2015.02.012, PP. 240-249

Keywords: YABBY家族,SlYABBY5,表达模式分析,非生物逆境胁迫

Full-Text   Cite this paper   Add to My Lib

Abstract:

?YABBY家族是植物中特有的一个小基因家族,具有典型N端C2C2型锌指结构域和C端螺旋环螺旋YABBY结构域。经同源序列比对分析,从番茄(SolanumLycopersicon)中克隆了YABBY家族的一个转录因子,将其命名为SlYABBY5(GenBank登录号为AK246138)。生物信息学分析表明,SlYABBY5基因ORF长为549bp,编码182个氨基酸残基,相对分子量为20.58kD,等电点为8.78。实时荧光定量PCR表达模式分析表明,SlYABBY5基因可能参与果实和叶片的发育;SlYABBY5在IAA、ABA、GA3、ASA和低温胁迫下表达量下调,在高盐和叶伤胁迫下表达量上调。研究结果初步证实SlYABBY5参与番茄的生长发育以及响应非生物逆境胁迫,为SlYABBY5基因参与逆境信号途径及番茄生长发育的研究提供了理论依据。

References

[1]  2.Siegfried K R,Eshed Y,Baum S F,et al.Members of the YABBY gene family specify abaxial cell fate in Arabidopsis[J].Development,1999,126(18):4117-4128.
[2]  3.Sawa S,Watanabe K,Goto K,et al.FILAMENTOUS FLOWER,a meristem and organ identity gene of Arabidopsis,encodes a protein with a zinc finger and HMG related domains[J].Genes Dev,1999,13:1079-1088.
[3]  4.Baxevanis A D,Landsman D.The Hmg-1 Box Protein Family-Classification and Functional-Relationships[J].Nucleic Acids Research,1995,23(9):1604-1613.
[4]  5.Bowman J L.The YABBY gene family and abaxial cell fate[J].Current Opinion in Plant Biology,2000,3(1):17-22.
[5]  6.Siegfried K R,Eshed Y,Baum S F,et al.Members of the YABBY gene family specify abaxial cell fate in Arabidopsis[J].Development,1999,126(18):4117-4128.
[6]  7.Stahle M I,Kuehlich J,Staron L,et al.YABBYs and the Transcriptional Corepressors LEUNIG and LEUNIG_HOMOLOG Maintain Leaf Polarity and Meristem Activity in Arabidopsis[J].Plant Cell,2009,21(10):3105-3118.
[7]  8.Sarojam R,Sappl P G,Goldshmidt A,et al.Differentiating Arabidopsis Shoots from Leaves by Combined YABBY Activities[J].Plant Cell,2010,22(7):2113-2130.
[8]  9.Bowman J L,Smyth D R.CRABS CLAW,a gene that regulates carpel and nectary development in Arabidopsis,encodes a novel protein with zinc finger and helix-loop-helix domains[J].Development,1999,126:2387-2396.
[9]  10.Villanueva J M,Broadhvest J,Hauser B A,et al.INNER NO OUTER regulates abaxial-adaxial patterning in Arabidopsis ovules[J].Genes Dev,1999,13:3160-3169.
[10]  11.Juarez M T,Twigg R W,Timmermans M C P.Specification of adaxial cell fate during maize leaf development[J].Development,2004,131(18):4533-4544.
[11]  12.Yamaguchi T,Nagasawa N,Kawasaki S,et al.The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa[J].Plant Cell,2004,16(2):500-509.
[12]  13.Dai M Q,Zhao Y,Ma Q F,et al.The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism[J].Plant Physiology,2007,144(1):121-133.
[13]  14.Dai M,Hu Y,Zhao Y,et al.A WUSCHEL- LIKE HOMEOBOX gene repressed a YABBY gene expression required for rice leaf development[J].Plant Physiol,2007,144(1):380-390.
[14]  15.Liu H L,Xu Y Y,Xu Z H,et al.A rice YABBY gene, OsYABBY4,preferentially expresses in developing vascular tissue[J].Dev Genes Evol,2007,217(9):629-637.
[15]  16.Zhao W,Su H Y,Song J,et al.Ectopic expression of TaYAB1,a member of YABBY gene family in wheat,causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis[J].Plant Sci,2006,170(2):364-371.
[16]  17.Toriba T,Harada K,Takamura A,et al.Molecular characterization the YABBY gene family in Oryza sativa and expression analysis of OsYABBY1[J].Molecular Genetics and Genomics,2007,277(5):457-468.
[17]  18.Takahiro Yamaguchi,Nobuhiro Nagasawa,Shinji Kawasaki,et al.The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa[J].The Plant Cell,2004,16,500-509.
[18]  19.Tanaka W,Toriba T,Ohmori Y,et al.The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet[J].Plant Cell ,2012,24(1):80-95.
[19]  20.Huang Z J,Van Houten J,Gonzalez G,et al.Genome-wide identification,phylogeny and expression analysis of SUN,OFP and YABBY gene family in tomato[J].Molecular Genetics and Genomics,2013,288(3-4):111-129.
[20]  21.Cong B,Barrero L S,Tanksley S D.Regulatory change in YABBY-like transcription factor led to evolution of extreme fruit size during tomato domestication[J].Nature Genetics,2008,40(6):800-804.
[21]  22.Bartley G E,Ishida B K.Digital fruit ripening:Data mining in the TIGR tomato gene index[J].Plant Molecular Biology Reporter,2002,20(2):115-130.
[22]  23.Bartley G E,Ishida B K.Developmental gene regulation during tomato fruit ripening and in-vitro sepal morphogenesis[J].BMC Plant Biology,2003,3:4.
[23]  24.Bartley G E,Ishida B K.Ethylene-sensitive and insensitive regulation of transcription factor expression during in vitro tomato sepal ripening[J].Journal of Experimental Botany,2007,58(8):2043-2051.
[24]  25.Minsung Kim,Thinh Pham1,Ashley Hamidi,et al.Reduced leaf complexity in tomato wiry mutants suggests a role for PHAN and KNOX genes in generating compound leaves[J].Development,2003,130,4405-4415.
[25]  26.Vrebalov J,Ruezinsky D,Padmanabhan V,et al.A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor( rin) locus[J].Science,2002,296(5566):343-346.
[26]  27.Wilkinson J Q,Lanahan M B,Yen H C,et al.An Ethylene-Inducible Component of Signal-Transduction Encoded by Never-Ripe[J].Science,1995,270(5243):1807-1809.
[27]  28.Vrebalov J,Pan I L,Arroyo A J M,et al.Fleshy Fruit Expansion and Ripening Are Regulated by the Tomato SHATTERPROOF Gene TAGL1[J].Plant Cell,2009,21(10):3041-3062.
[28]  29.张建苓,陈国平,朱明库,等.番茄DEAD-box RNA解旋酶基因 SlDEAH1的克隆及表达分析[J].植物研究,2014,34(1):44-52.
[29]  30.吴杨,贺俐,李伟,等.斑茅 δ-OAT基因克隆及序列分析[J].植物研究,2009,29(5):577-584.
[30]  31.Schneider M,Tognolli M,Bbiroch A.The Swiss-Protprotein knowledgebase and expasy:providing the plant community with high quality proteomic data and tools[J].Plant Physiol Biochem,2004(42):1013-1021.
[31]  32.Nakai K,Horton P.PSORT:a program for detecting sorting signals in proteins and predicting their suncelluar localization[J].Trends Biochem Sci,1999(24):34-36.
[32]  33.刘超,张林华,邱红林,等.天山雪莲 sikGLP基因的克隆及表达分析[J].植物研究,2013,33(4):461-467.
[33]  34.Exposito-Rodriguez M,Borges A A,Borges-Perez A,et al.Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process[J].Bmc Plant Biology,2008,8(1):131.
[34]  35.Hsieh T H,Li C W,Su R C,et al.A tomato bZIP transcription factor, SlAREB,is involved in water deficit and salt stress response[J].Planta,2010,231(6):1459-1473.
[35]  36.Yang M G,Yang Q Y,Fu T D,et al.Overexpression of the Brassica napus BnLAS gene in Arabidopsis affects plant development and increases drought tolerance[J].Plant Cell Reports,2011,30(3):373-388.
[36]  37.Exposito-Rodriguez M,Borges A A,Borges-Perez A,et al.Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process[J].Bmc Plant Biology,2008,8(1):131.
[37]  38.Hsieh T H,Li C W,Su R C,et al.A tomato bZIP transcription factor, SlAREB,is involved in water deficit and salt stress response[J].Planta,2010,231(6):1459-1473.
[38]  39.郭书巧,黄骥,江燕,等.水稻C2H2型锌指蛋白基因 RZF71的克隆与表达分析[J].遗传,2007,29(5):607-613.
[39]  40.莫晓婷,赵军,范云六,等.玉米转录因子结构与功能研究进展[J].中国农业科技导报,2013,15(3):7-17.
[40]  41.Jibran R,Hunter D A,Dijkwel P P.Hormonal regulation of leaf senescence through integration of developmental and stress signals[J].Plant Molecular Biology,2013,82(6):547-561.
[41]  42.李俊,傅兆麟.外源乙酰水杨酸对小麦灌浆期热害缓解效应的研究[J].扬州大学学报,2013,34(1):56-59.
[42]  43.Gepstein S,Thimann K V.Changes in the Abscisic-Acid Content of Oat Leaves during Senescence[J].Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences,1980,77(4):2050-2053.
[43]  44.Yang J C,Zhang J H,Wang Z Q,et al.Abscisic acid and cytokinins in the root exudates and leaves and their relationship to senescence and remobilization of carbon reserves in rice subjected to water stress during grain filling[J].Planta,2002,215(4):645-652.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133