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植物研究  2015 

两种短命植物HRD转录因子基因的克隆及序列分析

DOI: 10.7525/j.issn.1673-5102.2015.02.013, PP. 250-258

Keywords: 短命植物,HRD基因,转录因子,基因克隆,生物信息学分析

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Abstract:

?以新疆短命植物线果芥(ConringiaplanisiliquaL.)和大蒜芥(SisybriumaltissimumL.)为试验材料,采用同源序列克隆的方法,分别从线果芥和大蒜芥cDNA中克隆得到了一条HRD转录因子基因,命名为CpHRD和SaHRD。通过生物信息学分析,CpHRD和SaHRD转录因子基因开放阅读框长564bp,与山嵛菜(Eutremasalsugineum)、琴叶拟南芥(Arabidopsislyrata)、荠菜(Capsellarubella)及拟南芥(Arabidopsisthaliana)核苷酸序列的同源性分别为88%、87%、87%和86%,编码187个氨基酸,基因编码蛋白分子量约为20kD,二级结构包括2个β-折叠、5个a-螺旋和无规则卷曲,属含有信号肽的亲水性非跨膜稳定蛋白。亚细胞定位主要在细胞核中,系统进化树分析表明线果芥、大蒜芥和拟南芥属于同一种属,拥有1个典型的AP2/EREBP功能结构域,推测CpHRD和SaHRD基因属于AP2/EREBP转录因子家族EREBP亚组,可能参与植物ABA、干旱和低温等环境胁迫的应答过程,与植物的抗逆性有关。本研究为进一步深入探索HRD基因功能及其抗旱机制奠定了基础。研究同时也为短命植物种质资源的开发和利用发掘了新基因。

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