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植物研究  2015 

栽培小麦Brock白粉菌诱导早期应答基因SSH文库构建及分析

DOI: 10.7525/j.issn.1673-5102.2015.01.011, PP. 60-67

Keywords: 栽培小麦Brock,白粉病,抑制差减杂交,表达序列标签

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Abstract:

?以接种白粉菌的Brock叶片cDNA作为实验组(tester),不接菌Brock叶片cDNA为驱动组(driver),利用抑制性差减杂交技术,构建Brock白粉菌诱导早期应答基因消减杂交文库。随机挑选50个阳性克隆测序,获得38条非重复序列。经与GenBank进行同源比对,发现其中28条非重复序列与已知序列同源性较高。利用荧光定量PCR对部分基因分析后,发现ABC转运蛋白、丝/苏氨酸蛋白激酶、Lr1基因编码蛋白、核酮糖二磷酸羧基酶和叶绿素a/b结合蛋白参与了小麦抗白粉菌早期应答反应。该工作为我们在整体水平上揭示Brock抗白粉病分子机理提供重要依据。

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